He Ling, Lin Ye, Hu Xiulian, Zhang Yu, Wu Hui
Department of Implantology, School and Hospital of Stomatology, Peking University, Beijing, China.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Nov;108(5):707-13. doi: 10.1016/j.tripleo.2009.06.044.
The purpose of this study was to evaluate the effect of biologic characteristics of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) on proliferation and differentiation of rat osteoblasts.
Blood samples were collected from 14 healthy volunteers (7 male) with a mean age of 23.2 +/- 2.24 years. PRP and PRF were prepared with standard protocols. The exudates of PRP and PRF were collected at the time points of 1, 7, 14, 21, and 28 days. The levels of platelet-derived growth factor AB (PDGF-AB) and transforming growth factor beta1 (TGF-beta1) were quantified in PRP and PRF. Then the exudates of PRP and PRF were used to culture rat calvaria osteoblasts. The biologic characteristics of osteoblasts were analyzed in vitro for 14 days.
PRP released the highest amounts of TGF-beta1 and PDGF-AB at the first day, followed by significantly decreased release at later time points. PRF released the highest amount of TGF-beta1 at day 14 and the highest amount of PDGF-AB at day 7. Exudates of PRP collected at day 1 and exudates of PRF collected at day 14 expressed maximum alkaline phosphatase (ALP) activity, though no significance was shown. Cells treated with exudates of PRF collected at day 14 reached peak mineralization significantly more than both negative control and positive control groups. PRF is superior to PRP, from the aspects of expression of ALP and induction of mineralization.
PRF released autologous growth factors gradually and expressed stronger and more durable effect on proliferation and differentiation of rat osteoblasts than PRP in vitro.
本研究旨在评估富血小板血浆（PRP）和富血小板纤维蛋白（PRF）的生物学特性对大鼠成骨细胞增殖和分化的影响。
采集14名健康志愿者（7名男性）的血样，平均年龄为23.2±2.24岁。采用标准方案制备PRP和PRF。在第1、7、14、21和28天的时间点收集PRP和PRF的渗出液。对PRP和PRF中血小板衍生生长因子AB（PDGF-AB）和转化生长因子β1（TGF-β1）的水平进行定量。然后用PRP和PRF的渗出液培养大鼠颅骨成骨细胞。在体外分析成骨细胞的生物学特性，为期14天。
PRP在第一天释放的TGF-β1和PDGF-AB量最高，随后在后期时间点释放量显著下降。PRF在第14天释放的TGF-β1量最高，在第7天释放的PDGF-AB量最高。第1天收集的PRP渗出液和第14天收集的PRF渗出液表现出最大的碱性磷酸酶（ALP）活性，尽管未显示出显著性。用第14天收集的PRF渗出液处理的细胞比阴性对照组和阳性对照组显著更早达到矿化峰值。从ALP表达和矿化诱导方面来看，PRF优于PRP。
PRF在体外能逐渐释放自体生长因子，对大鼠成骨细胞的增殖和分化表现出比PRP更强且更持久的作用。
