Wu Baolin, Wei Na, Thon Vireak, Wei Mohui, Yu Zaikuan, Xu Yongmei, Chen Xi, Liu Jian, Wang Peng George, Li Tiehai
Center for Diagnostics and Therapeutics, Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA.
Org Biomol Chem. 2015 May 14;13(18):5098-101. doi: 10.1039/c5ob00462d.
A biotinylated heparosan hexasaccharide was synthesized using a one-pot multi-enzyme strategy, in situ activation and transfer of N-trifluoroacetylglucosamine (GlcNTFA) to a heparin backbone significantly improved the synthetic efficiency. The biotinylated hexasaccharide could serve as a flexible core to diversify its conversion into heparan sulfate isoforms with potential biological applications and therapeutics.
采用一锅多酶策略合成了生物素化的硫酸乙酰肝素六糖，将N-三氟乙酰葡糖胺（GlcNTFA）原位活化并转移至肝素主链显著提高了合成效率。该生物素化六糖可作为一个灵活的核心，用于多样化转化为具有潜在生物学应用和治疗用途的硫酸乙酰肝素异构体。
