Hoshino Y, Ichijo S, Osame S, Takahashi E
Nihon Juigaku Zasshi. 1989 Aug;51(4):741-8. doi: 10.1292/jvms1939.51.741.
For the purpose of clarifying the cause of white muscle disease (WMD) in calves, tocopherol and selenium levels and blood glutathione peroxidase (GSH-Px) activity were measured on 10 calves with WMD and nine of their dams. The main clinical symptoms of the 10 calves with WMD were motor disturbances including recumbency and stiffness. Serum enzyme activities (GOT, GPT, CPK, LDH) in calves with WMD increased markedly, and this increase was also observed in some of their dams. Serum tocopherol levels of calves with WMD were low, 70% of which showing deficient levels of less than 70 micrograms/100 ml. Serum selenium levels of all the calves were lower than 35 ppb, indicating a deficiency, and were accompanied by low blood GSH-Px activity. alpha-Tocopherol and selenium concentrations in organs were very low. Dams of calves with WMD showed low serum tocopherol levels, 22% of which indicating deficient levels below 150 micrograms/100 ml. Serum selenium levels in dams showed a marked decrease to under 20 ppb, and also low blood GSH-Px activity. Feedstuffs supplied in the farms to affected calves indicated very low alpha-tocopherol contents (below 3 mg/100g DM) and low selenium concentrations below 50 ppb in DM. It was concluded that WMD in calves was attributable to nutritional muscular dystrophy caused by deficiencies in tocopherol and selenium in feedstuffs supplied to their dams.
为了阐明犊牛白肌病（WMD）的病因，对10头患有白肌病的犊牛及其9头母牛的生育酚和硒水平以及血液谷胱甘肽过氧化物酶（GSH-Px）活性进行了测定。10头患有白肌病的犊牛的主要临床症状为运动障碍，包括卧地不起和僵硬。患有白肌病的犊牛血清酶活性（GOT、GPT、CPK、LDH）显著升高，在其部分母牛中也观察到了这种升高。患有白肌病的犊牛血清生育酚水平较低，其中70%显示低于70微克/100毫升的缺乏水平。所有犊牛的血清硒水平均低于35 ppb，表明缺乏，并伴有血液GSH-Px活性降低。器官中的α-生育酚和硒浓度非常低。患有白肌病的犊牛的母牛血清生育酚水平较低，其中22%表明低于150微克/100毫升的缺乏水平。母牛的血清硒水平显著下降至20 ppb以下，血液GSH-Px活性也较低。农场中供应给患病犊牛的饲料显示α-生育酚含量极低（低于3毫克/100克干物质），干物质中的硒浓度低于50 ppb。得出的结论是，犊牛白肌病归因于其母牛所供应饲料中生育酚和硒缺乏导致的营养性肌肉营养不良。
