Zhang Huining, Wang Hongyu, Yang Kai, Chang Qing, Sun Yuchong, Tian Jun, Long Chengli
School of Civil Engineering, Wuhan University, Wuhan 430072, China E-mail:
Northeast Electric Power Design Institute, Changchun 130000, China.
Water Sci Technol. 2015;71(7):1081-7. doi: 10.2166/wst.2015.071.
In the present study, a novel Pseudomonas sp. W1 was characterized in terms of its ability to perform nitrate removal coupled with anaerobic Fe⁻¹ oxidation under autotrophic growth condition. The effects of operating parameters with respect to the initial solution pH, temperature and initial Fe⁻¹ concentration on nitrate removal were investigated by central composite design. Based on the results of response surface methodology, the maximal nitrate removal efficiency was achieved under the following conditions: pH 7.0, temperature 30 °C and initial Fe⁻¹ concentration 1,100 mg L⁻¹. Under this optimal condition and with an initial NO(3)(-)-N concentration of 55 mg L⁻¹, this strain could remove NO(3)(-)-N with 90% reduction of NO(3)(-)-N, corresponding to oxidizing Fe⁻¹ with 71% oxidation of Fe⁻¹ after 7 days of incubation. The result of kinetic evaluation indicated that this bacterium showed significant substrate affinity to both NO(3)(-)-N and Fe⁻¹.
在本研究中，对一种新型假单胞菌属菌株W1进行了特性分析，该菌株在自养生长条件下具有同步去除硝酸盐和厌氧氧化亚铁的能力。采用中心复合设计研究了初始溶液pH值、温度和初始亚铁浓度等操作参数对硝酸盐去除的影响。基于响应面法的结果，在pH值7.0、温度30℃和初始亚铁浓度1100 mg/L的条件下实现了最大硝酸盐去除效率。在此最佳条件下，当初始硝酸盐氮浓度为55 mg/L时，该菌株在培养7天后可去除90%的硝酸盐氮，同时氧化71%的亚铁。动力学评估结果表明，该细菌对硝酸盐氮和亚铁均表现出显著的底物亲和力。
