Zhou Jun, Wang Hongyu, Yang Kai, Ji Bin, Chen Dan, Zhang Huining, Sun Yuchong, Tian Jun
School of Civil Engineering, Wuhan University, Wuhan, 430072, China.
School of Urban Construction, Wuhan University of Science and Technology, Wuhan, 430081, China.
Bioprocess Biosyst Eng. 2016 Feb;39(2):277-84. doi: 10.1007/s00449-015-1511-7. Epub 2015 Dec 9.
A continuous-upflow biofilter packed with sponge iron was constructed for nitrate removal under an anaerobic atmosphere. Microbacterium sp. W5, a nitrate reducing and Fe(II) oxidizing strain, was added to the biofilter as an inoculum. The best results were achieved when NO3 (-)-N concentration was 30 mg/L and Fe(2+) was 800 mg/L. Nitrite in influent would inhibit nitrate removal and aqueous Fe(2+) resulted in encrustation. Fe(II)EDTA would prevent cells from encrustation and the maximum nitrogen removal efficiency was about 90 % with Fe(II)EDTA level of 1100 mg/L. Nitrate reduction followed first-order reaction kinetics. Characteristics of biofilms were analyzed by X-ray fluorescence spectroscopy.
构建了一个填充海绵铁的连续上流式生物滤池，用于在厌氧气氛下去除硝酸盐。将一株具有硝酸盐还原和Fe（II）氧化能力的微杆菌属菌株W5作为接种物添加到生物滤池中。当NO3（-）-N浓度为30mg/L且Fe（2+）为800mg/L时，取得了最佳效果。进水亚硝酸盐会抑制硝酸盐的去除，而水溶液中的Fe（2+）会导致结垢。Fe（II）乙二胺四乙酸可防止细胞结垢，当Fe（II）乙二胺四乙酸水平为1100mg/L时，最大脱氮效率约为90%。硝酸盐还原遵循一级反应动力学。通过X射线荧光光谱分析生物膜的特性。
