用于检测燕窝中猪明胶的酶免疫测定法。
Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests.
作者信息
Tukiran Nur Azira, Ismail Amin, Mustafa Shuhaimi, Hamid Muhajir
机构信息
a Laboratory of Halal Science Research, Halal Products Research Institute , Universiti Putra Malaysia , UPM Serdang , Malaysia.
出版信息
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2015;32(7):1023-8. doi: 10.1080/19440049.2015.1039605. Epub 2015 Apr 29.
Porcine gelatine is a common adulterant found in edible bird's nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 µg g(-1), respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng µg(-1) (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry.
猪明胶是一种常见的掺入可食用燕窝中的 adulterant，用于在销售前增加净重。本研究旨在使用抗肽多克隆抗体制备用于检测猪明胶掺入情况的间接酶联免疫吸附测定（ELISA）。开发了三种间接 ELISA（PAB1、2 和 3），其检测限（LOD）分别为 0.12、0.10 和 0.11 μg g(-1)。当应用于猪明胶标准溶液时，批间和批内测定的变异系数（CV）均小于 20%，并且能够检测加标样品中至少 0.5 ng μg(-1)（0.05%）的猪明胶。所提出的 ELISA 为燕窝行业的质量控制提供了吸引力。 （注：“adulterant”此处结合语境推测可能是“掺杂剂”之类的意思，但在医学专业文献翻译中，可能需要结合更专业背景知识来准确理解其含义。）
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