新型里氏木霉 RH 7-13-9# 高产富马酸的木糖。
High production of fumaric acid from xylose by newly selected strain Rhizopus arrhizus RH 7-13-9#.
机构信息
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China; Amoy - BUCT Industrial Bio-technovation Institute, Amoy 361022, PR China.
出版信息
Bioresour Technol. 2015 Jun;186:348-350. doi: 10.1016/j.biortech.2015.03.109. Epub 2015 Mar 28.
Fumaric acid, as an important material for polymerization, is highly expected to be produced by fermentation of lignocellulosic biomass which is composed of cellulose, hemicellulose and lignin. Xylose as the main component of hemicellulose cannot be efficiently utilized by most of the common fermentation. In this study, a new strain Rhizopus arrhizus RH 7-13-9# was selected from the R. arrhizus RH 7-13 through a novel convenient and efficient selection method. Efficient production of fumaric acid (45.31 g/L) from xylose was achieved by the new strain, and the volumetric productivity was still 0.472 g/L h. Moreover, the conversion of xylose reached 73% which is close to the theoretic yield (77%). The production of fumaric acid was increased approximate by 172%, compared with the initial strain counterpart. These results indicated that xylose, as the main component of hemicellulose, has a promising application for the production of fumaric acid on an industrial-scale.
富马酸作为聚合反应的重要原料，有望通过纤维素、半纤维素和木质素组成的木质纤维素生物质发酵生产。半纤维素的主要成分木糖不能被大多数常见的发酵有效地利用。在这项研究中，通过一种新颖的方便有效的筛选方法，从根霉 RH7-13 中筛选到一种新的菌株 Rhizopus arrhizus RH7-13-9#。新菌株能够从木糖中高效生产富马酸（45.31 g/L），其比体积产率仍为 0.472 g/L h。此外，木糖的转化率达到 73%，接近理论产率（77%）。与原始菌株相比，富马酸的产量增加了约 172%。这些结果表明，木糖作为半纤维素的主要成分，有望在工业规模上用于生产富马酸。
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