Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China.
Bioresour Technol. 2013 Mar;131:303-7. doi: 10.1016/j.biortech.2012.12.148. Epub 2013 Jan 3.
An immobilization method using net was developed for fumaric acid fermentation by Rhizopus arrhizus RH-07-13. The large surface of the net immobilized enough filamentous mycelia which produced fumaric acid rapidly. Net size and spore concentration were optimized to enhance fermentation performance and 150cm(2) of net size, 0.5×10(6)per ml of spore concentration were selected finally. Compared to free-cell fermentation, fumaric acid production was flat (32.03 vs. 31.23g/L) but fermentation time reduced 83.3% (24 vs. 144h).
采用网兜固定化方法对米根霉 RH-07-13 进行延胡索酸发酵。网兜固定化方法可以为丝状真菌提供足够大的表面积,从而使其快速产生延胡索酸。通过优化网兜大小和孢子浓度,提高了发酵性能,最终选择网兜大小为 150cm(2),孢子浓度为 0.5×10(6)个/ml。与游离细胞发酵相比,延胡索酸的产量相当(32.03 与 31.23g/L),但发酵时间缩短了 83.3%(24 小时与 144 小时)。