Nakamura Yoshihiro, Miyaki Shigeru, Ishitobi Hiroyuki, Matsuyama Sho, Nakasa Tomoyuki, Kamei Naosuke, Akimoto Takayuki, Higashi Yukihito, Ochi Mitsuo
Department of Orthopedic Surgery, Hiroshima University, Hiroshima, Japan.
Department of Orthopedic Surgery, Hiroshima University, Hiroshima, Japan; Department of Regenerative Medicine, Hiroshima University Hospital, Hiroshima, Japan.
FEBS Lett. 2015 May 8;589(11):1257-65. doi: 10.1016/j.febslet.2015.03.031. Epub 2015 Apr 8.
Mesenchymal stem cell (MSC) transplantation is used for treatment of many diseases. The paracrine role of MSCs in tissue regeneration is attracting particular attention. We investigate the role of MSC exosomes in skeletal muscle regeneration. MSC exosomes promote myogenesis and angiogenesis in vitro, and muscle regeneration in an in vivo model of muscle injury. Although MSC exosomes had low concentrations of muscle-repair-related cytokines, a number of repair-related miRNAs were identified. This study suggests that the MSC-derived exosomes promote muscle regeneration by enhancing myogenesis and angiogenesis, which is at least in part mediated by miRNAs such as miR-494.
间充质干细胞（MSC）移植用于治疗多种疾病。MSC在组织再生中的旁分泌作用尤其受到关注。我们研究了MSC外泌体在骨骼肌再生中的作用。MSC外泌体在体外促进肌生成和血管生成，并在肌肉损伤的体内模型中促进肌肉再生。尽管MSC外泌体中与肌肉修复相关的细胞因子浓度较低，但仍鉴定出了一些与修复相关的miRNA。本研究表明，MSC来源的外泌体通过增强肌生成和血管生成来促进肌肉再生，这至少部分是由miR-494等miRNA介导的。
