van Dooren Ines, Faouzi My El Abbes, Foubert Kenn, Theunis Mart, Pieters Luc, Cherrah Yahya, Apers Sandra
Natural Products & Food Research and Analysis, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium.
Laboratory of Pharmacology, Faculty of Medicine and Pharmacy, Rabat-Institute, Al Karm street 11, Hay Riad, Rabat 10104, Morocco.
J Ethnopharmacol. 2015 Jul 1;169:69-75. doi: 10.1016/j.jep.2015.03.081. Epub 2015 Apr 9.
Infusions of Herniaria hirsuta L., Herniaria glabra L. and Herniaria fontanesii J.Gay are well known in Moroccon folk medicine for the treatment of biliary dyskinesia, (uro)lithiasis or as a diuretic. Herniariae Herba which can contain H. glabra and H. hirsuta is known in Europe as an urological drug.
To investigate the efficacy of a standardized infusion of Herniaria hirsuta against choleltihiasis, and evaluation of its genotoxicity.
An analytical HPLC-UV method to quantify flavonoids and saponins present in the extract of H. hirsuta was developed and validated. An in vivo experiment to evaluate the cholesterol lowering effect of a infusion of H. hirsuta in the gall bladder of dogs was carried out. Dogs were divided into 3 groups i.e. control dogs (CG), dogs treated with ursodeoxycholic acid (UDCA) (2×7.35mg/kg body weight/day) and dogs treated with the standardized infusion (HG) (2×48.5mg/kg body weight/day). Dogs were fed a fatty diet during 120 days after which a diet without additional fat was introduced till day 180. Treatment started 30 days after introduction of the fatty diet and lasted till the end of the experiment. A bile and blood sample of each dog was collected every 30 days, after which the concentration of cholesterol was determined. An Ames test was performed according to the OECD-guidelines.
The validated HPLC-UV method showed a linear calibration model and an acceptable precision for the total flavonoid content (total content 4.51%) as well as the total saponin content (12.74%). The in vivo experiments already showed a minor difference for bile cholesterol between CG and HG after 30 days of treatment with the infusion, and the difference was more pronounced after 90 days of treatment. Even 30 days after discontinuation of the cholesterol-rich diet a significant difference remained between CG and HG. There was no statistically significant difference in blood cholesterol. The Ames test showed that the infusion of H. hirsuta could be considered as being free from genotoxic risks.
A method for the standardization of a infusion of Herniaria hirsuta was developed and validated. Prolonged use of this standardized H. hirsuta extract resulted in a cholesterol-lowering effect in the bile of dogs. Since this pharmacological effect prevents the formation of gallstones and can contribute to solving existing gallstones, a standardized infusion of H. hirsuta may have a positive effect in the treatment of gallstones in human patients.
在摩洛哥民间医学中，多毛卷耳（Herniaria hirsuta L.）、光果卷耳（Herniaria glabra L.）和方氏卷耳（Herniaria fontanesii J.Gay）的浸剂因可用于治疗胆道运动障碍、（尿）结石症或作为利尿剂而广为人知。含有光果卷耳和多毛卷耳的卷耳草（Herniariae Herba）在欧洲作为一种泌尿科药物为人所知。
研究多毛卷耳标准化浸剂对胆石症的疗效，并评估其遗传毒性。
建立并验证了一种分析型高效液相色谱 - 紫外检测法，用于定量多毛卷耳提取物中的黄酮类化合物和皂苷。开展了一项体内实验，以评估多毛卷耳浸剂对犬胆囊中胆固醇降低作用。将犬分为3组，即对照犬（CG）、用熊去氧胆酸（UDCA）治疗的犬（2×7.35mg/kg体重/天）和用标准化浸剂治疗的犬（HG）（2×48.5mg/kg体重/天）。在120天内给犬喂食高脂饮食，之后引入无额外脂肪的饮食直至第180天。在引入高脂饮食30天后开始治疗，持续至实验结束。每30天采集每只犬的胆汁和血液样本，然后测定胆固醇浓度。根据经合组织指南进行艾姆斯试验。
验证后的高效液相色谱 - 紫外检测法显示，总黄酮含量（总含量4.51%）以及总皂苷含量（12.74%）具有线性校准模型和可接受的精密度。体内实验显示，在用浸剂治疗30天后，CG组和HG组之间胆汁胆固醇已有微小差异，治疗90天后差异更明显。即使在停止富含胆固醇饮食30天后，CG组和HG组之间仍存在显著差异。血液胆固醇方面无统计学显著差异。艾姆斯试验表明，多毛卷耳浸剂可被认为无遗传毒性风险。
建立并验证了一种多毛卷耳浸剂的标准化方法。长期使用这种标准化的多毛卷耳提取物可使犬胆汁中的胆固醇降低。由于这种药理作用可预防胆结石形成并有助于溶解现有的胆结石，多毛卷耳标准化浸剂可能对人类患者胆结石的治疗产生积极作用。
