Liu Lei, Gong Yu-Xin, Zhu Bin, Liu Guang-Lu, Wang Gao-Xue, Ling Fei
Northwest A&F University, Yangling 712100, People's Republic of China.
Northwest A&F University, Yangling 712100, People's Republic of China.
Fish Shellfish Immunol. 2015 Jul;45(1):175-83. doi: 10.1016/j.fsi.2015.03.043. Epub 2015 Apr 8.
Intestinal microbiota has become an integral component of the fish, and plays a key role in host metabolism, immunity and health maintenance. However, information on the immune responses after vaccine administration in relation to the intestinal microbiota is absent in fish. The present study focused on the effect of a new recombinant Aeromonas hydrophila vaccine (Aera) by using a novel functionalized, single-walled carbon nanotubes (SWCNTs) as a delivery vehicle on the intestinal microbiota of grass carp (Ctenopharyngodon idella) through the bath immunization, and further explored the immunological responses in intestine, kidney and spleen. By performing deep sequencing, a total of 81,979 valid reads and 609 OTUs obtained from 4 intestine samples were analyzed. We detected 141 genuses, most of which belonged to Firmicutes, Fusobacteria and Proteobacteria. Of note, the quantity of Aeromonas in library Aera (after 6 h Aera vaccine pretreatment, fish were transferred to tanks without SWCNTs-Aera for 28 d) and Aera-GD (6 h Aera vaccine pretreated prior to the group injected by A. hydrophila) was declined 6.5% and 14.6% compared with the control, respectively. Moreover, the expression of seven immune-related genes (IFN-I, TNF-α, CRP, IL-8, IgM, MHC I and CD8α) in the intestine, kidney and spleen of Aera treated fish was significantly enhanced, which indicated that a better tissue immune response in grass carp was induced by the SWCNTs-Aera vaccine. Therefore, a new recombinant SWCNT-Aera vaccine may represent potentially efficient and immunological role in grass carp intestine to resist A. hydrophila infection.
肠道微生物群已成为鱼类不可或缺的组成部分，并在宿主代谢、免疫和健康维持中发挥关键作用。然而，鱼类中关于疫苗接种后与肠道微生物群相关的免疫反应的信息尚不存在。本研究聚焦于一种新型重组嗜水气单胞菌疫苗（Aera）的效果，该疫苗使用新型功能化单壁碳纳米管（SWCNTs）作为递送载体，通过浸浴免疫对草鱼（Ctenopharyngodon idella）的肠道微生物群进行研究，并进一步探索肠道、肾脏和脾脏中的免疫反应。通过深度测序，对从4个肠道样本中获得的总共81,979条有效读数和609个OTU进行了分析。我们检测到141个属，其中大多数属于厚壁菌门、梭杆菌门和变形菌门。值得注意的是，文库Aera（Aera疫苗预处理6小时后，鱼转移到不含SWCNTs-Aera的水箱中28天）和Aera-GD（在嗜水气单胞菌注射组之前进行6小时Aera疫苗预处理）中嗜水气单胞菌的数量分别比对照组下降了6.5%和14.6%。此外，Aera处理的鱼的肠道、肾脏和脾脏中7个免疫相关基因（IFN-I、TNF-α、CRP、IL-8、IgM、MHC I和CD8α）的表达显著增强，这表明SWCNTs-Aera疫苗在草鱼中诱导了更好的组织免疫反应。因此，一种新型重组SWCNT-Aera疫苗可能在草鱼肠道中对抵抗嗜水气单胞菌感染具有潜在的高效性和免疫作用。
