基于流动夹心技术同时测定药物中的亚铁和抗坏血酸。

Simultaneous determination of iron (II) and ascorbic acid in pharmaceuticas based on flow sandwich technique.

作者信息

Vakh Christina, Freze Elena, Pochivalov Alexsey, Evdokimova Ekaterina, Kamencev Mihail, Moskvin Leonid, Bulatov Andrey

机构信息

Department of Analytical Chemistry, Institute of Chemistry, Saint Petersburg State University, Saint Petersburg, pr. Universitetskij 26, 198504, Russia.

出版信息

J Pharmacol Toxicol Methods. 2015 May-Jun;73:56-62. doi: 10.1016/j.vascn.2015.03.006. Epub 2015 Apr 8.

DOI:10.1016/j.vascn.2015.03.006

PMID:25862995

Abstract

The simple and easy performed flow system based on sandwich technique has been developed for the simultaneous separate determination of iron (II) and ascorbic acid in pharmaceuticals. The implementation of sandwich technique assumed the injection of sample solution between two selective reagents and allowed the carrying out in reaction coil two chemical reactions simultaneously: iron (II) with 1,10-phenanthroline and ascorbic acid with sodium 2,6-dichlorophenolindophenol. For achieving of excellent repeatability and considerable reagent saving the various parameters such as flow rate, sample and reagent volumes, reaction coil length were also optimized. The limits of detection (LODs) obtained by using the developed flow sandwich-type approach were 0.2 mg L(-1) for iron (II) and 0.7 mg L(-1) for ascorbic acid. The suggested approach was validated according to the following parameters: linearity and sensitivity, precision, recoveries and accuracy. The sampling frequency was 41 h(-1).

摘要

基于三明治技术开发了一种简单易行的流动系统，用于同时分别测定药物中的铁（II）和抗坏血酸。三明治技术的实施是将样品溶液注入两种选择性试剂之间，并允许在反应盘管中同时进行两个化学反应：铁（II）与1,10 - 菲咯啉反应，抗坏血酸与2,6 - 二氯酚靛酚钠反应。为了实现出色的重复性并大量节省试剂，还对诸如流速、样品和试剂体积、反应盘管长度等各种参数进行了优化。使用所开发的流动三明治型方法获得的检测限（LOD），铁（II）为0.2 mg L⁻¹，抗坏血酸为0.7 mg L⁻¹。根据以下参数对所建议的方法进行了验证：线性和灵敏度、精密度、回收率和准确度。采样频率为41 h⁻¹。