Karanfil Işıl, Bagci-Onder Tugba
Brain Cancer Research and Therapy Laboratory, Koç University School of Medicine, Rumelifeneri Yolu, Sarıyer, Istanbul, 34450, Turkey.
Methods Mol Biol. 2016;1357:329-38. doi: 10.1007/7651_2015_227.
Neural stem cells (NSCs) derived from induced pluripotent stem cells offer therapeutic tools for neurodegenerative diseases. This review focuses on embryoid body (EB)-mediated stem cell culture techniques used to derive NSCs from mouse induced pluripotent stem cells (iPSCs). Generation of healthy and stable NSCs from iPSCs heavily depends on standardized in vitro cell culture systems that mimic the embryonic environments utilized during neural development. Specifically, the neural induction and expansion methods after EB formation are described in this review.
源自诱导多能干细胞的神经干细胞为神经退行性疾病提供了治疗工具。本综述聚焦于用于从小鼠诱导多能干细胞（iPSC）中获得神经干细胞的拟胚体（EB）介导的干细胞培养技术。从iPSC中生成健康且稳定的神经干细胞在很大程度上依赖于模拟神经发育过程中所利用的胚胎环境的标准化体外细胞培养系统。具体而言，本综述描述了EB形成后的神经诱导和扩增方法。
