过氧化脲对多微生物生物膜模型的抗菌作用。

Antimicrobial effects of carbamide peroxide against a polymicrobial biofilm model.

作者信息

Hasegawa Haruhiko, Tomiyama Kiyoshi, Kumada Hidefumi, Kawata Akira, Higashi Kazuyoshi, Takahashi Osamu, Hamada Nobushiro, Mukai Yoshiharu

出版信息

Am J Dent. 2015 Feb;28(1):57-60.

PMID:25864244

Abstract

PURPOSE

To investigate the in vitro antimicrobial effects of carbamide peroxide (CP) and CP-based home bleaching agents against polymicrobial (PM) biofilms.

METHODS

Using a high-throughput active attachment model, PM biofilms were cultured on glass coverslips by diluting the stimulated saliva of one healthy adult. All experiments were performed anaerobically in McBain medium, which was refreshed twice daily. After biofilm formation for 24 or 72 hours, the biofilms were treated with 0.5%, 2.5%, 5%, or 10% CP, 20-fold dilutions of HiLite Shade Up (HS) or Opalescence Regular (OR), 0.2% chlorhexidine digluconate (CHX), 0.2% NaF, or deionized water (n = 10 each). Biofilms were dispersed and the number of colony forming units (CFU) was measured on tryptic soy agar blood plates. Coverslips containing 72-hour biofilms treated with 0.5% and 10% CP and deionized water were stained and scanned by confocal laser scanning microscopy (CLSM).

RESULTS

Treatment of 24- and 72-hour biofilms with HS, OR and CH yielded significantly fewer colonies than treatment with water or 0.2% NaF. No growing colonies were observed after treatment with 10% CP. CLSM showed that the percentage of dead bacteria increased as the concentration of CP increased.

摘要

目的

研究过氧化脲（CP）和基于CP的家庭漂白剂对多微生物（PM）生物膜的体外抗菌作用。

方法

采用高通量活性附着模型，通过稀释一名健康成年人的刺激唾液，在玻璃盖玻片上培养PM生物膜。所有实验均在McBain培养基中厌氧进行，每天更换两次培养基。生物膜形成24或72小时后，用0.5%、2.5%、5%或10%的CP、HiLite Shade Up（HS）或Opalescence Regular（OR）的20倍稀释液、0.2%葡萄糖酸洗必泰（CHX）、0.2%氟化钠或去离子水（每组n = 10）处理生物膜。将生物膜分散，在胰蛋白胨大豆琼脂血平板上测量菌落形成单位（CFU）的数量。用0.5%和10%的CP以及去离子水处理72小时生物膜的盖玻片，进行染色并通过共聚焦激光扫描显微镜（CLSM）扫描。