Meng Xiangrui, Sun Yuanyuan, Wang Lan, Li Yuhao, Ouyang Ruizhuo, Yuan Ping, Miao Yuqing
Institute of Bismuth Science, University of Shanghai for Science and Technology, Shanghai, China.
Department of Cardio-Pulmonary Circulation, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
Ann Transl Med. 2021 Jan;9(2):157. doi: 10.21037/atm-20-8137.
Under hypoxic conditions, the brain can undergo irreversible damage. The present study aimed to explore new higher-oxygen-content carbamide peroxide (CP) compounds and the effect of their oxygen-releasing property on human brain microvascular endothelial cell (EC) proliferation under hypoxic conditions.
Two different additives including alpha-terpineol and sorbic acid were added to the reaction system to obtain the carbamide peroxide of CP-I and CP-II.
We evaluated the oxygen generation capabilities of CP samples by using a portable dissolved oxygen meter. Consequently, alpha-terpineol as a stabilizer exhibited a unique effect on the oxygen generation of CP. CP-I was uniquely able to promote cell proliferation ability at 10 µg·L for hypoxic conditions, with the proliferation rates being 36.2% compared with the control group. The safety of CP to cells was further verified by calcein-AM/PI staining. Under hypoxic conditions, CP-I at 10 µg·L promoted the migration rate, and the migration rate being 32.37%.
These compounds have the advantages of simple synthesis, long storage time, low cost, and rich oxygen content. Used spectrophotometry, oxygen electrode test, and indicator titration for testing the oxygen production rate and oxygen production. The results indicate that alpha-terpineol is the best additive. CP-I exhibited the highest oxygen content and a superior effect on the cell phenotype than CP-II, especially under hypoxia. This study is the first to report the effects of CP on cells, and provides new therapeutic insights into cerebrovascular injury repair.
在低氧条件下，大脑会遭受不可逆损伤。本研究旨在探索新型高氧含量的过氧化脲（CP）化合物及其氧释放特性对低氧条件下人脑微血管内皮细胞（EC）增殖的影响。
向反应体系中添加两种不同的添加剂，即α-松油醇和山梨酸，以获得CP-I和CP-II的过氧化脲。
我们使用便携式溶解氧仪评估了CP样品的产氧能力。因此，α-松油醇作为稳定剂对CP的产氧具有独特作用。在低氧条件下，CP-I在10μg·L时能独特地促进细胞增殖能力，与对照组相比，增殖率为36.2%。通过钙黄绿素-AM/PI染色进一步验证了CP对细胞的安全性。在低氧条件下，10μg·L的CP-I促进了迁移率，迁移率为32.37%。
这些化合物具有合成简单、储存时间长、成本低和氧含量丰富的优点。使用分光光度法、氧电极测试和指示剂滴定法来测试产氧率和产氧量。结果表明，α-松油醇是最佳添加剂。CP-I表现出最高的氧含量，并且在细胞表型方面比CP-II具有更优的效果，尤其是在低氧条件下。本研究首次报道了CP对细胞的影响，并为脑血管损伤修复提供了新的治疗思路。
