Tanaka Kazuhisa, Shoji Atushi, Sugawara Masao
Department of Chemistry, College of Humanities and Sciences, Nihon University.
Anal Sci. 2015;31(4):321-5. doi: 10.2116/analsci.31.321.
An agarose gel slip containing L-glutamate oxidase (GluOx), horseradish peroxidase (HRP) and a dye DA-64 is proposed as a tool for visualizing ischemia-induced L-glutamate release in hippocampal slices in a flow system. The agarose slip with a detection limit of 6.0 ± 0.8 μmol L(-1) for L-glutamate enabled us to visualize L-glutamate fluxes in a flow system. The leak of a dye from the agarose gel was negligible and a diffusion blur due to spreading of Bindshedler's Green (BG) within the gel was suppressed. Monitoring the time-dependent change of ischemia-induced L-glutamate fluxes at neuronal regions CA1, DG and CA3 of hippocampal slices is demonstrated.
一种含有L-谷氨酸氧化酶（GluOx）、辣根过氧化物酶（HRP）和染料DA-64的琼脂糖凝胶片被提议作为一种工具，用于在流动系统中可视化海马切片中缺血诱导的L-谷氨酸释放。该琼脂糖片对L-谷氨酸的检测限为6.0±0.8μmol L(-1)，使我们能够在流动系统中可视化L-谷氨酸通量。染料从琼脂糖凝胶中的泄漏可忽略不计，并且抑制了由于Bindshedler绿（BG）在凝胶内扩散而导致的扩散模糊。展示了对海马切片神经元区域CA1、DG和CA3中缺血诱导的L-谷氨酸通量随时间变化的监测。
