Sinjari B, Diomede F, Murmura G, Traini T, Merciaro I, Trubiani O, Caputi S
Department of Medical, Oral and Biotechnological Sciences, University G. DAnnunzio, Chieti-Pescara, Italy.
J Biol Regul Homeost Agents. 2015 Jan-Mar;29(1):103-13.
Wound healing agents support the natural healing process, reduce trauma and likelihood of secondary infections and hasten wound closure. The aim of this work was to evaluate the effect of different concentration of a new Sardinian plant cream (RD7) on two human primary cultures: Periodontal Ligament Stem Cells (hPDLSCs) and Gingival Fibroblasts (hGFs) derived from oral tissues in terms of morphological changes, cell proliferation and wound healing properties. RD7, is an interactive dressing containing phytocomplex derived from Sardinian endemic or not, medicinal plant extracts, with an important anti-radical, anti-inflammatory and antiseptic activity finalized to rapidly promote tissue regeneration and the formation of granulation tissue. hPDLSCs and hGFs were seeded at different concentrations (0.5, 1, 2.5 and 5 mg/ml) of RD7. The cell proliferation and viability was evaluated using colorimetric assays (MTT assay) and trypan blue exclusion test. Meanwhile, the morphological cell changes were evaluated by means of optic (OM) and scanning electronic microscopes (SEM). The induction of the migratory properties was evaluated by means of wound healing assay. In vitro results, using hPDLSCs and hGFs, showed a decrease of cell growth starting at 24 h of incubation, at high concentrations (2.5 mg/ml and 5 mg/ml). This cell growth reduction was associated to evident morphological changes, whilst, at low concentrations (0.5 and 1 mg/ml) a typical unchanged morphology of both hPDLSCs and hGFs was shown. Wound healing assay showed a complete wound full closure occurring after 24 h of treatment in samples treated with low concentration of RD7. The results of the present work indicate that low concentrations of RD7 have no cytotoxicity effect, stimulate cell proliferation and contribute to induce the migratory properties in hPDLSCs and hGFs, therefore it could be considered a new product for use in clinical practice.
伤口愈合剂可支持自然愈合过程，减少创伤和二次感染的可能性，并加速伤口闭合。本研究的目的是评估一种新型撒丁岛植物乳膏（RD7）的不同浓度对两种人类原代培养物的影响：来自口腔组织的牙周膜干细胞（hPDLSCs）和牙龈成纤维细胞（hGFs），评估内容包括形态变化、细胞增殖和伤口愈合特性。RD7是一种交互式敷料，含有源自撒丁岛本土或非本土药用植物提取物的植物复合物，具有重要的抗自由基、抗炎和抗菌活性，旨在快速促进组织再生和肉芽组织形成。hPDLSCs和hGFs分别接种于不同浓度（0.5、1、2.5和5mg/ml）的RD7中。使用比色法（MTT法）和台盼蓝排斥试验评估细胞增殖和活力。同时，通过光学显微镜（OM）和扫描电子显微镜（SEM）评估细胞形态变化。通过伤口愈合试验评估迁移特性的诱导情况。使用hPDLSCs和hGFs的体外结果显示，在高浓度（2.5mg/ml和5mg/ml）下，孵育24小时后细胞生长开始下降。这种细胞生长减少与明显的形态变化相关，而在低浓度（0.5和1mg/ml）下，hPDLSCs和hGFs均显示出典型的形态未改变。伤口愈合试验表明，用低浓度RD7处理的样品在处理24小时后伤口完全闭合。本研究结果表明，低浓度的RD7没有细胞毒性作用，可刺激细胞增殖，并有助于诱导hPDLSCs和hGFs的迁移特性，因此可被视为一种用于临床实践的新产品。
