Department of Biomedical Sciences, Baylor College of Dentistry, Dallas, TX, USA.
Arch Oral Biol. 2011 Aug;56(8):812-22. doi: 10.1016/j.archoralbio.2011.01.001. Epub 2011 Apr 2.
Antioxidants (AOs) are the first line of defence against free radical damage and are critical for maintaining optimum health and well being. The need for AOs becomes even more critical with increased exposure to free radicals generated by pollution, cigarette smoke, drugs, illness, stress and exercise. Antioxidant supplementation is an excellent way of improving free radical protection. The aim of this study was to provide cytotoxicity, proliferation and migration data on the in vitro effects of bioactive AO mixtures on human oral fibroblasts.
Human oral fibroblasts were obtained from human gingival (HGF) and periodontal (HPDL) tissues. Each of these oral fibroblasts was cultured separately in three concentrations of the bioactive pure polyphenol and turmeric derivative mixtures; resveratrol (R), ferulic acid (F), phloretin (P) and tetrahydrocurcuminoids (T); [(RFT), (PFR), and (PFT)]. Cell viability, proliferation, morphology and migratory behaviour were analysed in vitro using high throughput in vitro 96 well plate wound assay.
RFT decreased (10(-3)M) and increased (10(-5)M) cell number in HGF cells. Three concentrations (10(-3), 10(-4), and 10(-5)M) of PFR and PFT increased DNA synthesis in HGF cells. PFT promoted cell migration but PFR and RFT had no significant change in HGF wound healing rates in a 96 well plate assay monolayer wound. In the HPDL cells, the 10(-4)M concentration of both RFT and PFT increased cell number at 72 h and 96 h whereas the lower concentration 10(-5)M of RFT significantly stimulated cell number at 96 h. PFR (10(-3)M and 10(-5)M) and PFT (10(-3)M) increased DNA synthesis after 48 h treatment in HPDL cells.
High and low concentrations (10(-3)-10(-5)M) of these AOs (RFT, PFR) may have beneficial effects on functional mechanisms regulating fibroblast migration and proliferation during gingival healing or periodontal repair.
抗氧化剂（AOs）是抵御自由基损伤的第一道防线，对于维持最佳健康和幸福至关重要。随着自由基生成的增加，抗氧化剂的需求变得更加关键，这些自由基是由污染、香烟烟雾、药物、疾病、压力和运动产生的。抗氧化剂补充是改善自由基保护的绝佳方法。本研究的目的是提供细胞毒性、增殖和迁移数据，以了解生物活性 AO 混合物对人口腔成纤维细胞的体外作用。
从人牙龈（HGF）和牙周（HPDL）组织中获得人口腔成纤维细胞。将每种口腔成纤维细胞分别在三种浓度的生物活性纯多酚和姜黄素衍生物混合物中培养；白藜芦醇（R）、阿魏酸（F）、根皮素（P）和四氢姜黄素（T）；[（RFT）、（PFR）和（PFT）]。使用高通量体外 96 孔板划痕试验在体外分析细胞活力、增殖、形态和迁移行为。
RFT（10（-3）M）减少和增加（10（-5）M）HGF 细胞中的细胞数。三种浓度（10（-3）、10（-4）和 10（-5）M）的 PFR 和 PFT 增加 HGF 细胞中的 DNA 合成。PFT 促进细胞迁移，但在 96 孔板测定单层划痕中，PFR 和 RFT 对 HGF 伤口愈合率没有显著变化。在 HPDL 细胞中，RFT 和 PFT 的 10（-4）M 浓度在 72 小时和 96 小时时增加细胞数量，而 RFT 的较低浓度 10（-5）M 显著刺激 96 小时时的细胞数量。PFR（10（-3）M 和 10（-5）M）和 PFT（10（-3）M）在 48 小时处理后增加 HPDL 细胞中的 DNA 合成。
这些 AOs（RFT、PFR）的高浓度和低浓度（10（-3）-10（-5）M）可能对调节牙龈愈合或牙周修复期间成纤维细胞迁移和增殖的功能机制具有有益作用。
