Li Xianglong, Han Pengfei, Guo Yingjia, Sun Huaiqiang, Xiao Ying, Kang Y James
Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
Department of Radiology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
J Neurosci Methods. 2015 Jul 15;249:59-65. doi: 10.1016/j.jneumeth.2015.04.004. Epub 2015 Apr 9.
Currently-used cerebellomedullary cistern puncture method for collecting cerebrospinal fluid (CSF) from monkeys is simple, inexpensive, and practical, but with high risk for brainstem injury and CSF blood contamination. An improved technique was thus developed and characterized.
Magnetic resonance imaging was used to identify the space and position of the cisterna magna in monkeys. Accordingly, a newly defined procedure for needle punctuation was tested in comparison with the traditional method. Blood contamination in CSF samples and brainstem injury were determined to define the superior of the improved method over the transitional method.
The cisterna magna in monkeys was found to be a "▽" shape. The needle was punctured into the cisterna magna aiming at the wider superior gap avoided brainstem injury. The improved method showed that the rate of blood contamination in the CSF samples was reduced from 66.7% to 16.7%, the higher rate of blood contamination was associated with higher risk for brainstem injury.
In traditional method, the needle is punctured aiming at the inferior gap with high density of blood vessels. In improved method, the needle is punctured aiming at the superior gap, pointing to the nose root while advancing the needle and avoiding injury to blood vessels.
This improved technique not only avoids blood contamination of CSF, but also prevents brainstem injury during the process of CSF collection. It is recommended for adaptation for CSF collection in monkeys.
目前用于从猴子采集脑脊液（CSF）的小脑延髓池穿刺方法简单、廉价且实用，但存在脑干损伤和脑脊液血液污染的高风险。因此，开发并表征了一种改进技术。
使用磁共振成像来识别猴子小脑延髓池的空间和位置。据此，与传统方法相比，测试了一种新定义的穿刺程序。通过测定脑脊液样本中的血液污染和脑干损伤情况来确定改进方法相对于传统方法的优势。
发现猴子的小脑延髓池呈“▽”形。将针穿刺入小脑延髓池时，瞄准较宽的上部间隙可避免脑干损伤。改进方法显示，脑脊液样本中的血液污染率从66.7%降至16.7%，较高的血液污染率与较高的脑干损伤风险相关。
在传统方法中，针瞄准血管密度高的下部间隙进行穿刺。在改进方法中，针瞄准上部间隙进行穿刺，进针时指向鼻根并避免损伤血管。
这种改进技术不仅避免了脑脊液的血液污染，还在脑脊液采集过程中防止了脑干损伤。推荐将其应用于猴子的脑脊液采集。
