College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.
College of Pharmacy, Harbin University of Commerce, Harbin, Heilongjiang, China.
Braz J Med Biol Res. 2021 Apr 26;54(6):e10032. doi: 10.1590/1414-431X202010032. eCollection 2021.
The objective of this study was to examine the safety of multiple repeated percutaneous punctures of cisterna magna for collecting cerebrospinal fluid (CSF) and preliminarily determine the optimal time interval and volume at each collection. Sixty Wistar rats were randomly assigned to six groups: 10 d-0 μL, 10 d-100 μL (100 μL CSF collected at an interval of 10 days), 10 d-150 μL, 15 d-0 μL, 15 d-100 μL, and 15 d-150 μL. CSF was collected by percutaneous puncture of the cisterna magna at four time-points. Simultaneously, locomotor activity, cisterna magna pressure, and acetylcholine levels in the CSF were monitored. Compared with the 10 d-0 μL group, the escape latency by Morris water maze was significantly prolonged in the 10 d-100 μL and 10 d-150 μL groups (P<0.05). Compared with the 15 d-0 μL group, the indices of 15 d-100 μL and 15 d-150 μL groups had no significant differences. When compared with that at the first training, the exception of the 10 d-150 μL and 15 d-150 μL groups, significant differences in escape latency were found at the 6th attempt (P<0.05). Compared with baseline readings for each group, the cisterna magna pressure in the 10 d-150 μL group began to decrease significantly from the third measurement (P<0.05). The optimal time interval during four CSF collections (100 μL per collection) via cisterna magna percutaneous puncture was determined to be 15 days. The procedure did not significantly affect learning processes, performance, or other related indices.
本研究旨在探讨多次重复经小脑延髓池穿刺抽取脑脊液（CSF）的安全性，并初步确定每次采集的最佳时间间隔和体积。60 只 Wistar 大鼠随机分为 6 组：10 d-0 μL、10 d-100 μL（10 天间隔采集 100 μL CSF）、10 d-150 μL、15 d-0 μL、15 d-100 μL 和 15 d-150 μL。通过小脑延髓池经皮穿刺采集 CSF 分四个时间点进行。同时监测运动活动、小脑延髓池压力和 CSF 中的乙酰胆碱水平。与 10 d-0 μL 组相比，10 d-100 μL 和 10 d-150 μL 组的 Morris 水迷宫逃避潜伏期明显延长（P<0.05）。与 15 d-0 μL 组相比，15 d-100 μL 和 15 d-150 μL 组的各项指标无显著差异。与第一次训练相比，除 10 d-150 μL 和 15 d-150 μL 组外，10 d-100 μL 和 15 d-100 μL 组的逃避潜伏期在第 6 次尝试时均有显著差异（P<0.05）。与各组基础读数相比，10 d-150 μL 组从第三次测量开始小脑延髓池压力明显下降（P<0.05）。经小脑延髓池经皮穿刺抽取 CSF（每次采集 100 μL）的四个最佳时间间隔为 15 天。该操作不会显著影响学习过程、表现或其他相关指标。
