Takikawa Megumi, Ishihara Masayuki, Takabayashi Yuki, Sumi Yuki, Takikawa Makoto, Yoshida Ryuichi, Nakamura Shingo, Hattori Hidemi, Yanagibayashi Satoshi, Yamamoto Naoto, Kiyosawa Tomoharu
a 1 Department of Plastic and Reconstructive Surgery, New Tokyo Hospital , Chiba, Japan.
b 2 Research Institute.
J Plast Surg Hand Surg. 2015 Oct;49(5):268-274. doi: 10.3109/2000656X.2015.1034723. Epub 2015 Apr 13.
The purpose of this study was to evaluate the accelerating effects of platelet-rich plasma-containing (PRP&) fragmin/protamine microparticles (F/P MPs) for repairing mitomycin C-treated healing-impaired wounds. Staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL-staining) showed that apoptosis of dermal fibroblast cells (DFCs) and epidermal keratinocyte cells (EKCs) were significantly induced in the skin of the mitomycin C-treated rats.
Full-thickness skin defects were made on the back of rats and mitomycin C was applied on the wounds to prepare a healing-impaired wound. After washing out the mitomycin C, saline (control), F/P MPs alone, PRP alone, and PRP&F/P MPs were injected around the wounds. The rats were later euthanised and histological sections of the wounds were then prepared at indicated time periods after the treatment.
These results indicated the numbers of large, medium, and small capillary lumens 7 days after injection of PRP&F/P MPs were significantly higher than those after injection of PRP or F/P MPs alone. Furthermore, epithelium and granulation tissue formations were significantly stimulated in the healing-impaired wounds treated with PRP&F/P MPs 3, 7 and 14 days after injection of PRP&F/P MPs.
本研究旨在评估含富血小板血浆（PRP）的达肝素/鱼精蛋白微粒（F/P MPs）对修复丝裂霉素C处理后愈合受损伤口的促进作用。末端脱氧核苷酸转移酶介导的dUTP缺口末端标记染色（TUNEL染色）显示，在丝裂霉素C处理的大鼠皮肤中，真皮成纤维细胞（DFCs）和表皮角质形成细胞（EKCs）的凋亡被显著诱导。
在大鼠背部制造全层皮肤缺损，并在伤口上应用丝裂霉素C以制备愈合受损伤口。冲洗掉丝裂霉素C后，在伤口周围注射生理盐水（对照）、单独的F/P MPs、单独的PRP以及PRP&F/P MPs。随后对大鼠实施安乐死，并在治疗后的指定时间段制备伤口的组织学切片。
这些结果表明，注射PRP&F/P MPs后7天，大、中、小毛细血管腔的数量显著高于单独注射PRP或F/P MPs后的数量。此外，在注射PRP&F/P MPs后3、7和14天，用PRP&F/P MPs处理的愈合受损伤口中的上皮和肉芽组织形成受到显著刺激。
