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组蛋白H1抑制体外组装的微型染色体的转录。

Histone H1 represses transcription from minichromosomes assembled in vitro.

作者信息

Shimamura A, Sapp M, Rodriguez-Campos A, Worcel A

机构信息

Department of Biology, University of Rochester, New York 14627.

出版信息

Mol Cell Biol. 1989 Dec;9(12):5573-84. doi: 10.1128/mcb.9.12.5573-5584.1989.

DOI:10.1128/mcb.9.12.5573-5584.1989
PMID:2586527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363727/
Abstract

We have previously shown that transcription from a Xenopus 5S rRNA gene assembled into chromatin in vitro can be repressed in the absence of histone H1 at high nucleosome densities (one nucleosome per 160 base pairs of DNA) (A. Shimamura, D. Tremethick, and A. Worcel, Mol. Cell. Biol. 8:4257-4269, 1988). We report here that transcriptional repression may also be achieved at lower nucleosome densities (one nucleosome per 215 base pairs of DNA) when histone H1 is present. Removal of histone H1 from the minichromosomes with Biorex under conditions in which no nucleosome disruption was observed led to transcriptional activation. Transcriptional repression could be restored by adding histone H1 back to the H1-depleted minichromosomes. The levels of histone H1 that repressed the H1-depleted minichromosomes failed to repress transcription from free DNA templates present in trans. The assembly of transcription complexes onto the H1-depleted minichromosomes protected the 5S RNA gene from inactivation by histone H1.

摘要

我们之前已经表明，在体外组装到染色质中的非洲爪蟾5S rRNA基因的转录，在高核小体密度（每160个碱基对DNA有一个核小体）且没有组蛋白H1的情况下会受到抑制（A. Shimamura、D. Tremethick和A. Worcel，《分子细胞生物学》8:4257 - 4269，1988年）。我们在此报告，当存在组蛋白H1时，在较低的核小体密度（每215个碱基对DNA有一个核小体）下也可实现转录抑制。在未观察到核小体破坏的条件下，用Biorex从小染色体上去除组蛋白H1会导致转录激活。通过将组蛋白H1重新添加到去除H1的小染色体上，可以恢复转录抑制。抑制去除H1的小染色体转录的组蛋白H1水平，无法抑制来自反式存在的游离DNA模板的转录。转录复合物在去除H1的小染色体上的组装，保护了5S RNA基因不被组蛋白H1灭活。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/66fa12734307/molcellb00060-0317-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/7705fc4e3173/molcellb00060-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/a2dc0d58745a/molcellb00060-0313-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/76f3dfd62828/molcellb00060-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/4a7ade702084/molcellb00060-0314-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/43880afc68a7/molcellb00060-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/e00e1d40f883/molcellb00060-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/66fa12734307/molcellb00060-0317-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/7705fc4e3173/molcellb00060-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/a2dc0d58745a/molcellb00060-0313-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/76f3dfd62828/molcellb00060-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/4a7ade702084/molcellb00060-0314-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/43880afc68a7/molcellb00060-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/e00e1d40f883/molcellb00060-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/845a/363727/66fa12734307/molcellb00060-0317-a.jpg

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