Day P, Shalaby Z, Cohen M M, Wasserman S S, Schwartz S
Department of Obstetrics/Gynecology, University of Maryland, School of Medicine, Baltimore 21201.
Mutat Res. 1989 Dec;224(4):409-13. doi: 10.1016/0165-1218(89)90065-7.
Frequencies of both sister-chromatid exchange (SCE) and chromosomal breakage (CB) were studied in the lymphocytes of normal individuals (10 and 7 individuals respectively). The cells were exposed in vitro to 3 different concentrations of theophylline (1, 10 and 100 micrograms/ml). A significant concentration effect of the drug was demonstrated for both SCEs and CB. Utilizing a Dunnett's test for individual comparisons, the 10 and 100 micrograms/ml concentrations both demonstrated a significant elevation of SCEs and CB compared to the untreated control cultures. This study suggests that in vitro concentrations of theophylline equal to or greater than 10 micrograms/ml, corresponding to serum levels attained during therapy, increase the frequency of SCEs and chromosome breakage in human lymphocytes.
在正常个体的淋巴细胞中研究了姐妹染色单体交换（SCE）和染色体断裂（CB）的频率（分别为10名和7名个体）。将细胞在体外暴露于3种不同浓度的茶碱（1、10和100微克/毫升）。对于SCE和CB均显示出该药物显著的浓度效应。利用Dunnett检验进行个体比较，与未处理的对照培养物相比，10和100微克/毫升浓度均显示出SCE和CB显著升高。本研究表明，体外等于或大于10微克/毫升的茶碱浓度，相当于治疗期间达到的血清水平，会增加人淋巴细胞中SCE和染色体断裂的频率。
