传统聚合酶链反应检测呼吸道合胞病毒的性能不佳——印度南部一家三级医疗中心的经验

Poor performance characteristics of conventional PCR in detection of respiratory syncytial virus-experience of a tertiary care centre in Southern India.

作者信息

Nandhini G, Sujatha S, Jain N, Dhodapkar R, Kadhiravan T, Krishnamurthy S

机构信息

Department of Microbiology, Jawaharlal Institute of Post Graduate Medical Education and Research, Puducherry - 605 006, India.

出版信息

Indian J Med Microbiol. 2015 Apr-Jun;33(2):274-6. doi: 10.4103/0255-0857.154875.

DOI:10.4103/0255-0857.154875

PMID:25865982

Abstract

Respiratory syncytial virus (RSV) is a significant cause of contagious acute respiratory infections in children and older adults. Since there are contradictory reports regarding the efficacy of different methods to detect RSV, we evaluated the performance of the conventional PCR versus real-time PCR in 222 patients with acute respiratory infections (ARI) recruited between January 2012 and March 2013. Conventional PCR had a very poor sensitivity of 40% (95% CI: 19.2-63.9%) and failed to detect RSV in respiratory samples with low viral load. Thus, it may be prudent to replace it with real-time PCR to achieve precise diagnosis.

摘要

呼吸道合胞病毒（RSV）是儿童和老年人传染性急性呼吸道感染的重要病因。由于关于检测RSV的不同方法的疗效存在相互矛盾的报道，我们评估了2012年1月至2013年3月招募的222例急性呼吸道感染（ARI）患者中传统PCR与实时PCR的性能。传统PCR的敏感性非常低，为40%（95%CI：19.2-63.9%），并且未能在病毒载量低的呼吸道样本中检测到RSV。因此，用实时PCR取代它以实现精确诊断可能是谨慎的做法。