Woo Seon Wook, Rhim Dong-Bin, Kim Changhee, Hwang Jae-Kwan
Department of Biotechnology, Yonsei University, Seoul 120-749, Korea.
Department of Biomaterials Science and Engineering, Yonsei University, Seoul 120-749, Korea.
Prev Nutr Food Sci. 2015 Mar;20(1):15-21. doi: 10.3746/pnf.2015.20.1.15. Epub 2015 Mar 31.
The skin plays a key role in protecting the body from the environment and from water loss. Cornified envelope (CE) and natural moisturizing factor (NMF) are considered as the primary regulators of skin hydration and barrier function. The CE prevents loss of water from the body and is formed by cross-linking of several proteins. Among these proteins, filaggrin is an important protein because NMF is produced by the degradation of filaggrin. Proteases, including matriptase and prostasin, stimulate the generation of filaggrin from profilaggrin and caspase-14 plays a role in the degradation of filaggrin. This study elucidated the effects of an ethanol extract of Boesenbergia pandurata (Roxb.) Schltr., known as fingerroot, and its active compound panduratin A on CE formation and filaggrin processing in HaCaT, human epidermal keratinocytes. B. pandurata extract (BPE) and panduratin A significantly stimulated not only CE formation but also the expression of CE proteins, such as loricrin, involucrin, and transglutaminase, which were associated with PPARα expression. The mRNA and protein levels of filaggrin and filaggrin-related enzymes, such as matriptase, prostasin, and caspase-14 were also up-regulated by BPE and panduratin A treatment. These results suggest that BPE and panduratin A are potential nutraceuticals which can enhance skin hydration and barrier function based on their CE formation and filaggrin processing.
皮肤在保护身体免受外界环境影响和防止水分流失方面发挥着关键作用。角质化包膜（CE）和天然保湿因子（NMF）被认为是皮肤水合作用和屏障功能的主要调节因子。CE可防止身体水分流失，由几种蛋白质交联形成。在这些蛋白质中，丝聚合蛋白是一种重要的蛋白质，因为NMF是由丝聚合蛋白降解产生的。包括膜型丝氨酸蛋白酶和前列腺素在内的蛋白酶刺激前丝聚合蛋白生成丝聚合蛋白，而半胱天冬酶-14在丝聚合蛋白的降解中起作用。本研究阐明了一种名为手指姜的闭鞘姜乙醇提取物及其活性化合物盘多苷A对人表皮角质形成细胞HaCaT中CE形成和丝聚合蛋白加工的影响。闭鞘姜提取物（BPE）和盘多苷A不仅显著刺激了CE的形成，还刺激了与PPARα表达相关的CE蛋白（如兜甲蛋白、内披蛋白和转谷氨酰胺酶）的表达。BPE和盘多苷A处理还上调了丝聚合蛋白以及丝聚合蛋白相关酶（如膜型丝氨酸蛋白酶、前列腺素和半胱天冬酶-14）的mRNA和蛋白质水平。这些结果表明，BPE和盘多苷A是潜在的营养保健品，基于它们对CE形成和丝聚合蛋白加工的作用，可增强皮肤水合作用和屏障功能。
