基于线粒体DNA COIII基因的藏牦牛群体遗传多样性与分类研究

Genetic diversity and classification of Tibetan yak populations based on the mtDNA COIII gene.

作者信息

Song Q Q, Chai Z X, Xin J W, Zhao S J, Ji Q M, Zhang C F, Ma Z J, Zhong J C

机构信息

Key Laboratory of Animal Genetics and Breeding, State Ethnic Affairs Commission and Ministry of Education, Southwest University for Nationalities, Chengdu, China.

Institute of Animal Science and Veterinary, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, China.

出版信息

Genet Mol Res. 2015 Mar 13;14(1):1763-70. doi: 10.4238/2015.March.13.3.

DOI:10.4238/2015.March.13.3

PMID:25867320

Abstract

To determine the level of genetic diversity and phylogenetic relationships among Tibetan yak populations, the mitochondrial DNA cytochrome c oxidase subunit 3 (COIII) genes of 378 yak individuals from 16 populations were analyzed in this study. The results showed that the length of cytochrome c oxidase subunit 3 gene sequences was 781 bp, with nucleotide frequencies of 29.2, 29.4, 26.1, and 15.2% for T, C, A, and G, respectively. A total of 26 haplotypes were identified, with 69 polymorphic sites, including 11 parsimony-informative sites and 58 single-nucleotide polymorphism sites. No deletions/insertions were found in sequence comparison, indicating that nucleotide mutation types were transitions and transversions. Haplotype and nucleotide diversities were 0.562 and 0.00138, respectively, indicating a high level of genetic diversity in Tibetan yak populations. Phylogenetic relationship analysis indicated that Tibetan yak populations are divided into 2 groups.

摘要

为了确定西藏牦牛群体的遗传多样性水平和系统发育关系，本研究分析了来自16个群体的378头牦牛个体的线粒体DNA细胞色素c氧化酶亚基3（COIII）基因。结果表明，细胞色素c氧化酶亚基3基因序列长度为781 bp，T、C、A和G的核苷酸频率分别为29.2%、29.4%、26.1%和15.2%。共鉴定出26种单倍型，有69个多态性位点，包括11个简约信息位点和58个单核苷酸多态性位点。序列比对未发现缺失/插入，表明核苷酸突变类型为转换和颠换。单倍型多样性和核苷酸多样性分别为0.562和0.00138，表明西藏牦牛群体具有较高的遗传多样性。系统发育关系分析表明，西藏牦牛群体分为2组。