Zhang J D, Yang Q
College of Life Science and Technology, Harbin Institute of Technology, Harbin, China.
College of Life Science and Technology, Harbin Institute of Technology, Harbin, China
Genet Mol Res. 2015 Mar 13;14(1):1771-81. doi: 10.4238/2015.March.13.4.
The aim of this study was to develop a protocol for the production of fungal bio-pesticides with high efficiency, low cost, and non-polluting fermentation, while also increasing their survival rate under field conditions. This is the first study to develop biocontrol Trichoderma harzianum transformants TS1 that are resistant to benzimidazole fungicides. Agricultural corn stover and wheat bran waste were used as a medium and inducing carbon source for solid fermentation. Spore production was observed, and the method was optimized using single-factor tests with 4 factors at 3 levels in an orthogonal experimental design to determine the optimal culture conditions for T. harzianum TS1. In this step, we determined the best conditions for fermenting the biocontrol fungi. The optimal culture conditions for T. harzianum TS1 were cultivated for 8 days, a ratio of straw to wheat bran of 1:3, ammonium persulfate as the nitrogen source, and a water content of 30 mL. Under optimal culture conditions, the sporulation of T. harzianum TS1 reached 1.49 x 10(10) CFU/g, which was 1.46-fold higher than that achieved before optimization. Increased sporulation of T. harzianum TS1 results in better utilization of space and nutrients to achieve control of plant pathogens. This method allows for the recycling of agricultural waste straw.
本研究的目的是制定一种生产高效、低成本且无污染发酵的真菌生物农药的方案，同时提高其在田间条件下的存活率。这是首次开发对苯并咪唑类杀菌剂具有抗性的生防哈茨木霉转化体TS1的研究。农业玉米秸秆和麦麸废弃物被用作固体发酵的培养基和诱导碳源。观察了孢子产生情况，并采用正交试验设计，对4个因素在3个水平上进行单因素试验，优化方法以确定哈茨木霉TS1的最佳培养条件。在这一步中，我们确定了发酵生防真菌的最佳条件。哈茨木霉TS1的最佳培养条件为培养8天，秸秆与麦麸比例为1:3，以过硫酸铵为氮源，含水量为30 mL。在最佳培养条件下，哈茨木霉TS1的产孢量达到1.49×10(10) CFU/g，比优化前提高了1.46倍。哈茨木霉TS1产孢量的增加有利于更好地利用空间和养分以实现对植物病原菌的控制。这种方法能够实现农业废弃秸秆的循环利用。
