Du X Y, Yi S, Huo X Y, Wang C, Liu D F, Ren W Z, Chen Z W
Department of Laboratory Animal Science, Capital Medical University, Beijing, China.
Laboratory Animal Center, Jilin University, Changchun, China.
Genet Mol Res. 2015 Mar 13;14(1):1788-97. doi: 10.4238/2015.March.13.6.
We optimized a panel of microsatellite markers from cat and tiger genetic data for efficient genetic monitoring and used it to analyze the genetic structure of an outbred cat stock in China. We selected a set of rich polymorphic microsatellite loci from 131 cat microsatellite loci and 3 Sumatran tiger microsatellite loci using agarose gel electrophoresis. Next, the set of optimized genetic markers was used to analyze the genetic variation in an outbred population of orange tabby cats in China by simple-tandem repeat scanning. Thirty-one loci rich in polymorphisms were selected and the highest allele number in a single locus was 8. Analysis of the orange tabby cat population illustrated that the average observed number of alleles, mean effective allele number, mean Shannon's information index, mean expected heterozygosity, and observed heterozygosity were 3.8387, 2.4027, 0.9787, 0.5565, and 0.5528, respectively. The 31 microsatellite markers used were polymorphic and suitable for analyzing the genetic structure of cats. The population of orange tabby cats was confirmed to be a well-outbred stock.
我们从猫和老虎的遗传数据中优化了一组微卫星标记，用于高效的遗传监测，并利用其分析了中国一个远交猫种群的遗传结构。我们通过琼脂糖凝胶电泳从131个猫微卫星位点和3个苏门答腊虎微卫星位点中筛选出一组具有丰富多态性的微卫星位点。接下来，利用这组优化后的遗传标记，通过单链重复扫描分析了中国橙色虎斑猫远交群体的遗传变异。共筛选出31个多态性丰富的位点，单个位点的最高等位基因数为8。对橙色虎斑猫群体的分析表明，平均观察等位基因数、平均有效等位基因数、平均香农信息指数、平均期望杂合度和观察杂合度分别为3.8387、2.4027、0.9787、0.5565和0.5528。所使用的31个微卫星标记具有多态性，适用于分析猫的遗传结构。橙色虎斑猫群体被确认为一个良好的远交种群。
