Sun Weibin, Chen Hong, Lei Chuzhao, Lei Xueqin, Zhang Yinghan
College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China.
J Genet Genomics. 2007 Jan;34(1):17-25. doi: 10.1016/S1673-8527(07)60003-4.
To evaluate the genetic polymorphisms and to search for available molecular markers for Qinchuan cattle, 90 Qinchuan cows were genotyped with 12 microsatellite markers. A total of 247 alleles were detected, with the number of alleles ranging from 13 (INRA005) to 33 (HEL13), giving a mean number of 21 alleles per locus. The total and mean effective allele number were 142.6229 and 11.8852, respectively. Mean sampling variance of the allele frequency was 2.6036 x 10(-4). Allele size ranges of the 12 microsatellite loci were different. The observed heterozygosity and expected heterozygosity were from 0.7842 (INRA005) to 0.9775 (BM315) and 0.7952 (BM315) to 0.9446 (HEL13), respectively. Mean observed heterozygosity and mean expected heterozygosity were 0.9117 and 0.9047, respectively. Polymorphism information content values were from 0.7653 (INRA005) to 0.9420 (HEL13), and mean polymorphism information content of the 12 microsatellite loci was 0.8965. All the 12 microsatellite loci were highly polymorphic, which showed that there were rich genetic polymorphisms at these detected microsatellite loci in Qinchuan cows. At the 12 microsatellite loci, the mean fixation index was -0.0076, reflecting that the degree of heterozygote defect at these loci was not high and deviations from Hardy-Weinberg equilibrium were not significant.
为评估秦川牛的遗传多态性并寻找可用的分子标记，用12个微卫星标记对90头秦川母牛进行基因分型。共检测到247个等位基因，等位基因数从13个（INRA005）到33个（HEL13）不等，每个位点的平均等位基因数为21个。总有效等位基因数和平均有效等位基因数分别为142.6229和11.8852。等位基因频率的平均抽样方差为2.6036×10(-4)。12个微卫星位点的等位基因大小范围不同。观察杂合度和期望杂合度分别为0.7842（INRA005）至0.9775（BM315）和0.7952（BM315）至0.9446（HEL13）。平均观察杂合度和平均期望杂合度分别为0.9117和0.9047。多态信息含量值为0.7653（INRA005）至0.9420（HEL13），12个微卫星位点的平均多态信息含量为0.8965。所有12个微卫星位点均具有高度多态性，表明在这些检测到的微卫星位点上，秦川母牛存在丰富的遗传多态性。在12个微卫星位点上，平均固定指数为-0.0076，反映出这些位点的杂合子缺陷程度不高，与哈迪-温伯格平衡的偏差不显著。
