Moreno-Ramírez L, González-Mendoza D, Cecena-Duran C, Grimaldo-Juarez O
Instituto de Ciencias Agrícolas, Universidad Autónoma de Baja California, Baja California, México.
Instituto de Ciencias Agrícolas, Universidad Autónoma de Baja California, Baja California, México
Genet Mol Res. 2015 Mar 31;14(1):2793-8. doi: 10.4238/2015.March.31.9.
One of the main limitations in intensive crop production in Northwestern Mexico is the dependence on the use of phosphate fertilizer. In this study, we isolated indigenous microorganisms with phosphate solubilization capacities from mesquite (Prosopis glandulosa) present in the Mexicali valley. In total, 4 bacteria were isolated from the rhizosphere of mesquite, including ICA01, ICA02Ba, ICA03Bs, and ICA04Ma. The bacterial isolates were identified based on their phenotypic and 16S rRNA gene sequencing data to be Acinetobacter calcoaceticus. The results showed that ICA01 was the most efficient in solubilizing phosphate, followed by ICA02Ba and ICA03Bs, while ICA04Ma showed the lowest phosphate-solubilizing activity. The pH value of the culture medium decreased with bacterial growth, suggesting that these strains produce organic acids that solubilize phosphorus. These results will be useful for biotechnological studies and A. calcoaceticus may be employed for biofertilization programs in northwest Mexico.
墨西哥西北部集约化作物生产的主要限制因素之一是对磷肥使用的依赖。在本研究中，我们从墨西卡利山谷的牧豆树（Prosopis glandulosa）中分离出具有解磷能力的本土微生物。总共从牧豆树的根际分离出4株细菌，包括ICA01、ICA02Ba、ICA03Bs和ICA04Ma。根据其表型和16S rRNA基因测序数据，将分离出的细菌鉴定为醋酸钙不动杆菌。结果表明，ICA01在解磷方面效率最高，其次是ICA02Ba和ICA03Bs，而ICA04Ma的解磷活性最低。培养基的pH值随着细菌生长而降低，表明这些菌株产生了溶解磷的有机酸。这些结果将有助于生物技术研究，醋酸钙不动杆菌可用于墨西哥西北部的生物施肥计划。
