Das Devsmita, Phillips Cristy, Lin Bill, Mojabi Fatemeh, Akif Baktir Mehmet, Dang Van, Ponnusamy Ravikumar, Salehi Ahmad
VA Palo Alto Health Care System; Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine.
Department of Physical Therapy, Arkansas State University.
J Vis Exp. 2015 Mar 31(97):52371. doi: 10.3791/52371.
Dendritic arborization has been shown to be a reliable marker for examination of structural and functional integrity of neurons. Indeed, the complexity and extent of dendritic arborization correlates well with the synaptic plasticity in these cells. A reliable method for assessment of dendritic arborization is needed to characterize the deleterious effects of neurological disorders on these structures and to determine the effects of therapeutic interventions. However, quantification of these structures has proven to be a formidable task given their complex and dynamic nature. Fortunately, sophisticated imaging techniques can be paired with conventional staining methods to assess the state of dendritic arborization, providing a more reliable and expeditious means of assessment. Below is an example of how these imaging techniques were paired with staining methods to characterize the dendritic arborization in wild type mice. These complementary imaging methods can be used to qualitatively and quantitatively assess dendritic arborization that span a rather wide area within the hippocampal region.
树突分支已被证明是检查神经元结构和功能完整性的可靠标志物。事实上，树突分支的复杂性和范围与这些细胞中的突触可塑性密切相关。需要一种可靠的方法来评估树突分支，以表征神经疾病对这些结构的有害影响，并确定治疗干预的效果。然而，鉴于这些结构复杂且动态的性质，对其进行量化已被证明是一项艰巨的任务。幸运的是，先进的成像技术可以与传统染色方法相结合，以评估树突分支的状态，提供一种更可靠、更快捷的评估方法。以下是这些成像技术如何与染色方法相结合来表征野生型小鼠树突分支的一个例子。这些互补的成像方法可用于定性和定量评估海马区域内相当大范围内的树突分支。
