Yan Jiajie, Thomson Justin K, Zhao Weiwei, Fast Vladimir G, Ye Tong, Ai Xun
Department of Cell and Molecular Physiology, Loyola University Chicago.
Department of Biomedical Engineering, University of Alabama at Birmingham.
J Vis Exp. 2015 Mar 23(97):52542. doi: 10.3791/52542.
Optical mapping has proven to be a valuable technique to detect cardiac electrical activity on both intact ex vivo hearts and in cultured myocyte monolayers. HL-1 cells have been widely used as a 2-Dimensional cellular model for studying diverse aspects of cardiac physiology. However, it has been a great challenge to optically map calcium (Ca) transients and action potentials simultaneously from the same field of view in a cultured HL-1 atrial cell monolayer. This is because special handling and care is required to prepare healthy cells that can be electrically captured and optically mapped. Therefore, we have developed an optimal working protocol for dual channel optical mapping. In this manuscript, we have described in detail how to perform the dual channel optical mapping experiment. This protocol is a useful tool to enhance the understanding of action potential propagation and Ca kinetics in arrhythmia development.
光学映射已被证明是一种在完整的离体心脏和培养的心肌细胞单层中检测心脏电活动的有价值技术。HL-1细胞已被广泛用作研究心脏生理学各个方面的二维细胞模型。然而，在培养的HL-1心房细胞单层中，从同一视野同时光学映射钙（Ca）瞬变和动作电位一直是一个巨大的挑战。这是因为需要特殊的处理和护理来制备能够被电捕获和光学映射的健康细胞。因此，我们开发了一种用于双通道光学映射的最佳工作方案。在本手稿中，我们详细描述了如何进行双通道光学映射实验。该方案是增强对心律失常发生过程中动作电位传播和钙动力学理解的有用工具。
