Yang ZhiPing, Sun JianMing, Xu Zhe
a Dalian Huixin Titanium Equipment Development Company, Ltd., Youjia Industrial Garden Xinzhaizi Town , Ganjingzi District, Dalian 116039 , China.
J Aquat Anim Health. 2015 Jun;27(2):71-6. doi: 10.1080/08997659.2014.993483.
The purpose of this study was to evaluate the effects of dietary administration of the live yeast, Rhodotorula sp. C11, on growth and disease resistance against Vibrio splendidus infection in juvenile Japanese spiky sea cucumber Apostichopus japonicus. Sea cucumbers were fed diets containing Rhodotorula sp. C11 at 0 (control), 10⁴, 10⁵, and 10⁶ CFU/g of feed for 45 d. There were three replicate tanks per dietary treatment. The specific growth rates were higher in all sea cucumbers treated with Rhodotorula sp. C11 than in the controls. Following a challenge with V. splendidus NB13, the cumulative prevalence and mortality of sea cucumbers fed diets supplemented with Rhodotorula sp. C11 were lower than in animals fed the basal diet. In sea cucumbers fed diets supplemented with Rhodotorula sp. C11 for 42 d, the only viable yeast found in the intestine was Rhodotorula sp. C11, which had counts of 1.58-1.98 × 10⁴CFU/g. No yeast was isolated from the intestine of animals fed the basal diet. For the colonization study, 20 sea cucumbers from each dietary treatment were removed to separate tanks and fed the control diet from day 16 to day 46. The viable yeast (Rhodotorula sp. C11) counts in the intestine decreased to 60-80 CFU/g by day 37. Moreover, as demonstrated by denaturing gradient gel electrophoresis, Rhodotorula sp. C11 colonization of the intestine could be detected until day 46. The differences in culture and PCR-denaturing gradient gel electrophoresis may be due to differences in the sensitivity of both methods. The present result showed that Rhodotorula sp. C11 was able to successfully colonize the intestine of juvenile Japanese spiky sea cucumbers by dietary supplementation, which improved its growth and disease resistance.
本研究旨在评估日粮添加活酵母红酵母属C11对日本刺参幼参生长及抵抗灿烂弧菌感染的抗病力的影响。将刺参投喂含0（对照）、10⁴、10⁵和10⁶CFU/g饲料的红酵母属C11日粮45天。每个日粮处理设置三个重复养殖池。所有经红酵母属C11处理的刺参的特定生长率均高于对照组。在用灿烂弧菌NB13攻毒后，日粮添加红酵母属C11的刺参的累积发病率和死亡率低于投喂基础日粮的动物。在投喂添加红酵母属C11日粮42天的刺参中，肠道中唯一可培养的酵母是红酵母属C11，其数量为1.58 - 1.98×10⁴CFU/g。在投喂基础日粮的动物肠道中未分离到酵母。对于定殖研究，从每个日粮处理中选取20只刺参转移至单独的养殖池中，从第16天至第46天投喂对照日粮。到第37天时，肠道中可培养酵母（红酵母属C11）数量降至60 - 80 CFU/g。此外，变性梯度凝胶电泳结果表明，直至第46天仍可检测到红酵母属C11在肠道中的定殖。培养法和PCR - 变性梯度凝胶电泳结果的差异可能是由于两种方法灵敏度不同所致。目前的结果表明，通过日粮添加，红酵母属C11能够成功定殖于日本刺参幼参肠道，从而改善其生长和抗病力。
