Hamedi Azadeh, Farjadian Shirin, Karami Mohammad Reza
Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
J Evid Based Complementary Altern Med. 2015 Oct;20(4):269-74. doi: 10.1177/2156587215580490. Epub 2015 Apr 12.
Taranjebin manna is a substance produced by Poophilus nebulosus Leth. (Aphrophoridae) larva that feed from host plant Alhagi maurorum (Leguminosae). In Persian ethnomedicine, it is used as an antipyretic, antiviral, antimicrobial, demulcent, and adaptogen. But it is contraindicated in acute fever and some infections. This controversy might be due to its immunomodulatory properties. This study evaluated immunomodulatory properties of Taranjebin and its macromolecules. Taranjebin solution was prepared as described in traditional literature. After dialysis and precipitation, the macromolecules were isolated on DEAE Sephadex A-25. The cytotoxic/proliferative properties of Taranjebin and its isolated macromolecules on human Jurkat E6.1 cells were investigated (15.62-1000 μg/mL) using WST-1 reagent. Three of 4 isolated acidic polysaccharides inhibited the proliferation of Jurkat cells in a dose-dependent manner at concentrations higher than 31.25 μg/mL (IC50 range of 44.81-147.97 μg/mL). The crude aqueous Taranjebin solution had proliferative effects. These results indicate the immunomodulatory properties of Taranjebin.
塔拉杰宾甘露是由嗜沫蝉（沫蝉科）的幼虫产生的一种物质，该幼虫以寄主植物骆驼刺（豆科）为食。在波斯民族医学中，它被用作退烧药、抗病毒药、抗菌药、缓和剂和适应原。但在急性发热和某些感染中禁用。这种争议可能归因于其免疫调节特性。本研究评估了塔拉杰宾及其大分子的免疫调节特性。按照传统文献中的描述制备塔拉杰宾溶液。经过透析和沉淀后，在DEAE葡聚糖A-25上分离大分子。使用WST-1试剂研究了塔拉杰宾及其分离出的大分子对人Jurkat E6.1细胞的细胞毒性/增殖特性（15.62 - 1000μg/mL）。4种分离出的酸性多糖中的3种在浓度高于31.25μg/mL时以剂量依赖性方式抑制Jurkat细胞的增殖（IC50范围为44.81 - 147.97μg/mL）。粗制的塔拉杰宾水溶液具有增殖作用。这些结果表明塔拉杰宾具有免疫调节特性。
