Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon 210-702, Republic of Korea.
Int J Biol Macromol. 2012 Dec;51(5):720-9. doi: 10.1016/j.ijbiomac.2012.07.006. Epub 2012 Jul 13.
Sulfated polysaccharides isolated from Capsosiphon fulvescens and fractionated using ion-exchange chromatography were investigated to determine their chemical and molecular characteristics and biological activities. The crude and fractionated polysaccharides (F(1), F(2), and F(3)) consisted mostly of carbohydrates (28.9-67.0%), uronic acids (1.6-9.2%) and sulfates (5.2-13.4%) with various amounts of proteins (2.1-53.7%). Their monosaccharide levels were significantly different including rhamnose (20.8-65.2%), xylose (13.0-37.1%) and mannose (11.6-65.1%). The polysaccharides contained one or two subfractions with molecular weights (M(w)) ranging from 401.7×10(3) to 6232×10(3) g/mol. These polysaccharides (the crude and fraction F(2)) strongly stimulated macrophage cells, RAW264.7 cell line, producing considerable amounts of NO, PGE(2) and cytokines which suggested that they could be strong immunostimulators. The main backbone of the most immunoenhancing polysaccharide (F(2)) was suggested by GC-MS and NMR to be the following: [formula, see text].
从金顶侧耳中分离出的硫酸多糖，并通过离子交换色谱进行分级，以确定其化学和分子特征以及生物活性。粗多糖和分级多糖（F1、F2 和 F3）主要由碳水化合物（28.9-67.0%）、糖醛酸（1.6-9.2%）和硫酸盐（5.2-13.4%）组成，还有不同量的蛋白质（2.1-53.7%）。它们的单糖水平明显不同，包括鼠李糖（20.8-65.2%）、木糖（13.0-37.1%）和甘露糖（11.6-65.1%）。多糖含有一个或两个亚组分，分子量（Mw）范围为 401.7×103-6232×103g/mol。这些多糖（粗多糖和分级 F2）强烈刺激巨噬细胞 RAW264.7 细胞系产生大量的 NO、PGE2 和细胞因子，这表明它们可能是强大的免疫刺激剂。最具免疫增强作用的多糖（F2）的主要骨架通过 GC-MS 和 NMR 被确定为：[公式，见正文]。
