Zhang Xiao-Hui, Liu Zhao-Zhao, Tang Mao-Xing, Zhang Yong-Hong, Hu Lian, Liao Ai-Hua
Family Planning Research Institute, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Family Planning Research Institute, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China
Reprod Sci. 2015 Nov;22(11):1377-86. doi: 10.1177/1933719115580999. Epub 2015 Apr 12.
To establish a mouse model for endometrial injury and determine the underlying mechanism regarding its favorable effect on embryo implantation.
Female Kunming mice were randomly allocated into 4 groups: group I, normal control; group II, injury procedure control; and group III and group IV, the mice being scratched with a blunt syringe on the right uterine horn or both, respectively. All the mice were mated with the males during the next estrus phase. The number of implanted embryos on each side of uterus was calculated on day 8 of pregnancy. The endometrial samples were taken on day 4 of pregnancy, and the local morphological changes and cytokine expressions were examined.
Compared to group II, our results showed that in group IV (1) there were significantly higher numbers of implanted embryos, (2) the endometrial glands and vasculatures in stroma were obviously increased and the pinopodes were abundant and well developed, and (3) the local levels of cytokines leukemia inhibitory factor (LIF) and oncostatin M (OSM) messenger RNA and protein expression were significantly increased.
Local mechanical injury on mouse uteri enhanced endometrial receptivity and improved embryo implantation, which were correlated with the characteristic changes in endometrial morphology and the upregulation of LIF and OSM gene and protein expression.
建立子宫内膜损伤小鼠模型，并确定其对胚胎着床产生有利影响的潜在机制。
将雌性昆明小鼠随机分为4组：I组为正常对照组；II组为损伤操作对照组；III组和IV组分别用钝头注射器刮擦右侧子宫角或双侧子宫角。所有小鼠在下一个发情期与雄性小鼠交配。在妊娠第8天计算子宫两侧着床胚胎的数量。在妊娠第4天采集子宫内膜样本，检查局部形态变化和细胞因子表达。
与II组相比，我们的结果显示IV组（1）着床胚胎数量显著增加，（2）基质中的子宫内膜腺体和血管明显增多，微绒毛丰富且发育良好，（3）细胞因子白血病抑制因子（LIF）和制瘤素M（OSM）的信使核糖核酸及蛋白表达的局部水平显著升高。
小鼠子宫局部机械损伤增强了子宫内膜容受性并改善了胚胎着床，这与子宫内膜形态的特征性变化以及LIF和OSM基因及蛋白表达上调相关。
