Maniatsi Stefania, Farmaki Theodora, Abatzopoulos Theodore J
Department of Genetics, Development & Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 541 24, Greece.
Institute of Applied Biosciences (IN. A.B.), Centre for Research and Technology - HELLAS, 6th klm. Charilaou-Thermi Rd., Thermi, Thessaloniki 57001, Greece.
Comp Biochem Physiol B Biochem Mol Biol. 2015 Aug;186:8-19. doi: 10.1016/j.cbpb.2015.04.002. Epub 2015 Apr 11.
Research on stress responses in animals has increased greatly during the last decades. Though most studies focus on the cellular and molecular bases of the stress response mechanisms, the ecological and evolutionary aspects of stress responses gain more and more interest. Here, we use species and parthenogenetic strains of the genus Artemia, an extremophile model organism, to study, for the first time, a protein well known for its chaperone activity and its involvement in stress responses. More specifically, transcription and protein accumulation of an FK506-Binding Protein (FKBP) homologue were investigated under heat and salt stresses. Additionally, the mRNA levels of ubiquitin, a heat-inducible protein related to the proteasomal pathway, were quantitated under these conditions. Biochemical and phylogenetic analyses showed that the studied FKBP orthologue is a typical representative of the family that clusters with other crustacean sequences. The expression was increased in both fkbp and ubiquitin genes after salt and heat stresses. However, our results in combination with the fact that Artemia species and parthenogenetic strains, selected for this study, exhibit different heat or salt tolerance provide useful hints about the evolutionary significance of FKBP and ubiquitin. Regarding FKBP, mRNA expression and protein accumulation seem to depend on the environmental conditions and the evolutionary history of each Artemia population while ubiquitin has a clear and more conserved role under heat shock.
在过去几十年中，对动物应激反应的研究大幅增加。尽管大多数研究集中在应激反应机制的细胞和分子基础上，但应激反应的生态和进化方面越来越受到关注。在这里，我们使用卤虫属的物种和孤雌生殖品系，一种极端嗜盐生物模型，首次研究一种以其伴侣活性和参与应激反应而闻名的蛋白质。更具体地说，在热应激和盐应激下研究了FK506结合蛋白（FKBP）同源物的转录和蛋白质积累。此外，在这些条件下对泛素（一种与蛋白酶体途径相关的热诱导蛋白）的mRNA水平进行了定量。生化和系统发育分析表明，所研究的FKBP直系同源物是该家族的典型代表，与其他甲壳类序列聚类。盐应激和热应激后，fkbp和泛素基因的表达均增加。然而，我们的结果与本研究中选择的卤虫物种和孤雌生殖品系表现出不同的耐热性或耐盐性这一事实相结合，为FKBP和泛素的进化意义提供了有用的线索。关于FKBP，mRNA表达和蛋白质积累似乎取决于每个卤虫种群的环境条件和进化历史，而泛素在热休克下具有明确且更保守的作用。
