Sawada K, Caballé-Serrano J, Schuldt Filho G, Bosshardt D D, Schaller B, Buser D, Gruber R
Department of Cranio-maxillofacial Surgery, Inselspital, University of Bern, Bern, Switzerland; Laboratory of Oral Cell Biology, School of Dental Medicine, University of Bern, Bern, Switzerland.
Laboratory of Oral Cell Biology, School of Dental Medicine, University of Bern, Bern, Switzerland; Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland; Department of Oral and Maxillofacial Surgery, College of Dentistry, Universitat Internacional de Catalunya, Barcelona, Spain.
Int J Oral Maxillofac Surg. 2015 Aug;44(8):1060-6. doi: 10.1016/j.ijom.2015.03.012. Epub 2015 Apr 11.
The autoclaving, pasteurization, and freezing of bone grafts to remove bacteria and viruses, and for preservation, respectively, is considered to alter biological properties during graft consolidation. Fresh bone grafts release paracrine-like signals that are considered to support tissue regeneration. However, the impact of the autoclaving, pasteurization, and freezing of bone grafts on paracrine signals remains unknown. Therefore, conditioned medium was prepared from porcine cortical bone chips that had undergone thermal processing. The biological properties of the bone-conditioned medium were assessed by examining the changes in expression of target genes in oral fibroblasts. The data showed that conditioned medium obtained from bone chips that had undergone pasteurization and freezing changed the expression of adrenomedullin, pentraxin 3, BTB/POZ domain-containing protein 11, interleukin 11, NADPH oxidase 4, and proteoglycan 4 by at least five-fold in oral fibroblasts. Bone-conditioned medium obtained from autoclaved bone chips, however, failed to change the expression of the respective genes. Also, when bone-conditioned medium was prepared from fresh bone chips, autoclaving blocked the capacity of bone-conditioned medium to modulate gene expression. These in vitro results suggest that pasteurization and freezing of bone grafts preserve the release of biologically active paracrine signals, but autoclaving does not.
对骨移植材料进行高压灭菌、巴氏消毒和冷冻，分别用于去除细菌和病毒以及保存，但这些操作被认为会在移植材料融合过程中改变其生物学特性。新鲜骨移植材料会释放类似旁分泌的信号，这些信号被认为有助于组织再生。然而，骨移植材料的高压灭菌、巴氏消毒和冷冻对旁分泌信号的影响尚不清楚。因此，我们从经过热处理的猪皮质骨碎片中制备了条件培养基。通过检测口腔成纤维细胞中靶基因表达的变化，评估了骨条件培养基的生物学特性。数据显示，从经过巴氏消毒和冷冻的骨碎片中获得的条件培养基，可使口腔成纤维细胞中肾上腺髓质素、五聚素3、含BTB/POZ结构域蛋白11、白细胞介素11、NADPH氧化酶4和蛋白聚糖4的表达变化至少五倍。然而，从高压灭菌的骨碎片中获得的骨条件培养基未能改变相应基因的表达。此外，当从新鲜骨碎片制备骨条件培养基时，高压灭菌会阻碍骨条件培养基调节基因表达的能力。这些体外实验结果表明，骨移植材料的巴氏消毒和冷冻可保留生物活性旁分泌信号的释放，但高压灭菌则不能。
