Shahraki Mohammad, Mohammadi Rahim, Najafpour Alireza
Researcher, Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Assistant Professor, Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
J Oral Maxillofac Surg. 2015 Jul;73(7):1438.e1-9. doi: 10.1016/j.joms.2015.03.032. Epub 2015 Mar 19.
FK506 is an immunosuppressant agent used to prevent rejection after organ transplantation. The aim of the present study was to assess effects of tacrolimus (FK506) on peripheral nerve regeneration using allografts in a rat sciatic nerve model.
Thirty male white Wistar rats were divided randomly into a normal control (NC) group (n = 10), an allograft (ALLO) group (n = 10), and an FK506-treated (ALLO/FK506) group (n = 10). In the NC group, the left sciatic nerve was exposed through a gluteal muscle incision and, after homeostasis, the muscle was sutured. In the ALLO group, the left sciatic nerve was exposed through a gluteal muscle incision and transected proximal to the tibioperoneal bifurcation, where a 10-mm segment was excised. The same procedure was performed in the ALLO/FK506 group. The harvested nerves of the ALLO group served as allografts for the ALLO/FK506 group and vice versa. The NC and ALLO groups received sterile olive oil 300 μL intraperitoneally once a day for 1 week and the ALLO/FK506 group received FK506 300 μL (1 mg/kg) intraperitoneally once a day for 1 week.
Behavioral, functional, and biomechanical recovery and gastrocnemius muscle mass showed earlier regeneration of axons in the ALLO/FK506 than in the ALLO group (P < .05). Histomorphometric and immunohistochemical studies also showed earlier regeneration of axons in the ALLO/FK506 than in the ALLO group (P < .05).
Administration of FK506 could accelerate functional recovery of the sciatic nerve after nerve allografting. It could have clinical implications for the surgical management of patients after facial nerve transection.
FK506是一种免疫抑制剂，用于预防器官移植后的排斥反应。本研究的目的是在大鼠坐骨神经模型中评估他克莫司（FK506）对同种异体神经移植后周围神经再生的影响。
30只雄性白色Wistar大鼠随机分为正常对照组（NC组，n = 10）、同种异体移植组（ALLO组，n = 10）和FK506治疗组（ALLO/FK506组，n = 10）。NC组通过臀肌切口暴露左侧坐骨神经，止血后缝合肌肉。ALLO组通过臀肌切口暴露左侧坐骨神经，在胫腓骨分叉近端横断，切除10 mm节段。ALLO/FK506组进行相同操作。ALLO组收获的神经作为ALLO/FK506组的同种异体移植物，反之亦然。NC组和ALLO组每天腹腔注射300 μL无菌橄榄油，共1周；ALLO/FK506组每天腹腔注射300 μL FK506（1 mg/kg），共1周。
行为学、功能学、生物力学恢复以及腓肠肌质量显示，ALLO/FK506组轴突再生早于ALLO组（P < 0.05）。组织形态计量学和免疫组织化学研究也显示，ALLO/FK506组轴突再生早于ALLO组（P < 0.05）。
给予FK506可加速神经同种异体移植后坐骨神经的功能恢复。这可能对面神经横断患者的手术治疗具有临床意义。
