Manaflouyan Khajehmarjany Soheila, Rahmani Seyed Ali, Chavoshi Seyed Hadi, Esfahani Ali, Movassaghpour Akbari Ali Akbar
Department of Cellular and Molecular Biology, Islamic Azad University, Ahar Branch, East Azerbaijan, Iran ; Dr. Rahmani Medical Genetic Lab, Tabriz, East Azerbaijan, Iran.
Department of Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, East Azerbaijan, Iran ; Emam Reza Hospital, Tabriz, East Azerbaijan, Iran.
Cell J. 2015 Spring;17(1):171-80. doi: 10.22074/cellj.2015.525. Epub 2015 Apr 8.
Chronic myeloid leukemia (CML) is a myeloproliferative disease. The cytogenetic hallmark of CML is Philadelphia (Ph) chromosome. This study aimed to diagnose suspected CML patients, to monitor CML patients under therapy using cytogenetic and fluorescence in situ hybridization (FISH) techniques to analyze their bone marrow (BM) and peripheral blood (PB) samples, and finally to compare their obtained results for both specimens. This study was conducted during one-year period (2012-2013). The participants were recruited from the Hematology and Oncology Clinic of Shahid Gazi (Emam Reza) Hospital of Tabriz University of Medical Sciences, Tabriz, East Azerbaijan Province, Iran. We analyzed 90 samples from 60 suspected CML patients (30 BM and 60 PB samples). All samples were analyzed using G-banding, 5 samples using dual fusion FISH (DF-FISH) probes, as well as 30 samples using both FISH and G-banding. Among the 90 analyzed samples of 60 patients, 25 (41.66%) were Ph+ using karyotyping, whereas five cases were not analyzable, so FISH was applied and the results confirmed that only two individuals were BCR-ABL+. In the comparison between 25 BM and 25 PB samples using karyotyping, 15 (60%) and 10 (40%) were ph+, respectively. The comparison of FISH and karyotyping on 30 samples showed that 9 (30%) and 8 (26.66%) were Ph+, respectively, and only 18.18% of Ph+ patients showed atypical patterns. In the comparison between BM-cytogenetic and PB- interphase-FISH (I-FISH), BM-cytogenetic was more reliable than PB-I-FISH in detecting Ph. Our data demonstrate that FISH analysis is a rapid, reliable and sensitive technique. The comparison between BM and PB showed that PB can not be replaced by BM, even in detecting by FISH.
慢性髓性白血病（CML）是一种骨髓增殖性疾病。CML的细胞遗传学标志是费城（Ph）染色体。本研究旨在诊断疑似CML患者，使用细胞遗传学和荧光原位杂交（FISH）技术分析其骨髓（BM）和外周血（PB）样本，以监测接受治疗的CML患者，最后比较两种标本的检测结果。本研究在一年期间（2012 - 2013年）进行。参与者来自伊朗东阿塞拜疆省大不里士医科大学沙希德加齐（伊玛目礼萨）医院的血液学和肿瘤学诊所。我们分析了60例疑似CML患者的90份样本（30份BM样本和60份PB样本）。所有样本均采用G显带分析，5份样本采用双融合FISH（DF - FISH）探针分析，30份样本同时采用FISH和G显带分析。在60例患者的90份分析样本中，25份（41.66%）核型分析为Ph +，而5例无法分析，因此采用FISH检测，结果证实只有2例为BCR - ABL +。在使用核型分析比较25份BM样本和25份PB样本时，Ph +分别为15份（60%）和10份（40%）。对30份样本进行FISH和核型分析比较显示，Ph +分别为9份（30%）和8份（26.66%），仅18.18%的Ph +患者表现出非典型模式。在BM细胞遗传学与PB间期FISH（I - FISH）的比较中，BM细胞遗传学在检测Ph方面比PB - I - FISH更可靠。我们的数据表明，FISH分析是一种快速、可靠且灵敏的技术。BM与PB的比较表明，即使在FISH检测中，PB也不能被BM替代。
