Le Gouill S, Talmant P, Milpied N, Daviet A, Ancelot M, Moreau P, Harousseau J L, Bataille R, Avet-Loiseau H
Laboratory of Hematology and Clinical Hematology Department, University Hospital, Nantes, France.
J Clin Oncol. 2000 Apr;18(7):1533-8. doi: 10.1200/JCO.2000.18.7.1533.
To evaluate the usefulness of fluorescence in situ hybridization (FISH) on peripheral-blood specimens to evaluate the cytogenetic response to treatment in patients with chronic myeloid leukemia (CML).
In a first attempt, we analyzed 62 bone marrow specimens using interphase FISH and compared the results with those of conventional cytogenetics. In a second step, we analyzed 60 paired sets of bone marrow and peripheral-blood specimens with interphase FISH.
The results of interphase FISH agreed with conventional cytogenetics on bone marrow for most patients, and only minor differences were found (r =.98). The comparison of interphase FISH on bone marrow versus peripheral-blood specimens showed a strong correlation between these two specimen sources (r =.97).
Our results confirmed that FISH is a sensitive technique for the evaluation of response to treatment in patients with CML. Moreover, our study suggests that follow-up of cytogenetic response to therapy can be evaluated on peripheral-blood specimens, thus enabling an easier and more frequent evaluation of patients. The next step will be to evaluate this technique in a large prospective trial to define the prognostic value of complete remissions evaluated by FISH.
评估荧光原位杂交（FISH）在外周血标本中对慢性髓性白血病（CML）患者治疗的细胞遗传学反应进行评估的实用性。
首先，我们使用间期FISH分析了62份骨髓标本，并将结果与传统细胞遗传学结果进行比较。其次，我们用间期FISH分析了60对骨髓和外周血标本。
对于大多数患者，间期FISH结果与骨髓的传统细胞遗传学结果一致，仅发现微小差异（r = 0.98）。骨髓与外周血标本的间期FISH比较显示这两种标本来源之间具有很强的相关性（r = 0.97）。
我们的结果证实FISH是评估CML患者治疗反应的一种敏感技术。此外，我们的研究表明，可以对外周血标本进行治疗的细胞遗传学反应随访，从而能够更轻松、更频繁地评估患者。下一步将在大型前瞻性试验中评估该技术，以确定通过FISH评估的完全缓解的预后价值。
