Liu Bin, Sun Zheng, Ma Xiaonian, Yang Bo, Jiang Yue, Wei Dong, Chen Feng
School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China.
Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China.
Int J Mol Sci. 2015 Apr 13;16(4):8201-12. doi: 10.3390/ijms16048201.
Extracellular polysaccharides (EPS) produced by marine microalgae have the potential to be used as antioxidants, antiviral agents, immunomodulators, and anti-inflammatory agents. Although the marine microalga Crypthecodinium cohnii releases EPS during the process of docosahexaenoic acid (DHA) production, the yield of EPS remains relatively low. To improve the EPS production, a novel mutagenesis of C. cohnii was conducted by atmospheric and room temperature plasma (ARTP). Of the 12 mutants obtained, 10 mutants exhibited significantly enhanced EPS yield on biomass as compared with the wild type strain. Among them, mutant M7 was the best as it could produce an EPS volumetric yield of 1.02 g/L, EPS yield on biomass of 0.39 g/g and EPS yield on glucose of 94 mg/g, which were 33.85%, 85.35% and 57.17% higher than that of the wild type strain, respectively. Results of the present study indicated that mutagenesis of the marine microalga C. cohnii by ARTP was highly effective leading to the high-yield production of EPS.
海洋微藻产生的胞外多糖（EPS）有潜力用作抗氧化剂、抗病毒剂、免疫调节剂和抗炎剂。尽管海洋微藻寇氏隐甲藻在二十二碳六烯酸（DHA）生产过程中会释放EPS，但EPS的产量仍然相对较低。为了提高EPS产量，利用常压室温等离子体（ARTP）对寇氏隐甲藻进行了新型诱变。在获得的12个突变体中，与野生型菌株相比，10个突变体的EPS产量在生物量上显著提高。其中，突变体M7表现最佳，其EPS体积产量为1.02 g/L，基于生物量的EPS产量为0.39 g/g，基于葡萄糖的EPS产量为94 mg/g，分别比野生型菌株高33.85%、85.35%和57.17%。本研究结果表明，通过ARTP对海洋微藻寇氏隐甲藻进行诱变非常有效，可实现EPS的高产。
