Zhang Xue, Zhang Xiao-Fei, Li He-Ping, Wang Li-Yan, Zhang Chong, Xing Xin-Hui, Bao Cheng-Yu
Department of Chemical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China.
Appl Microbiol Biotechnol. 2014 Jun;98(12):5387-96. doi: 10.1007/s00253-014-5755-y. Epub 2014 Apr 27.
Developing rapid and diverse microbial mutation tool is of importance to strain modification. In this review, a new mutagenesis method for microbial mutation breeding using the radio-frequency atmospheric-pressure glow discharge (RF APGD) plasma jets is summarized. Based on the experimental study, the helium RF APGD plasma jet has been found to be able to change the DNA sequences significantly, indicating that the RF APGD plasma jet would be a powerful tool for the microbial mutagenesis with its outstanding features, such as the low and controllable gas temperatures, abundant chemically reactive species, rapid mutation, high operation flexibility, etc. Then, with the RF APGD plasma generator as the core component, a mutation machine named as atmospheric and room temperature plasma (ARTP) mutation system has been developed and successfully employed for the mutation breeding of more than 40 kinds of microorganisms including bacteria, fungi, and microalgae. Finally, the prospect of the ARTP mutagenesis is discussed.
开发快速且多样的微生物突变工具对于菌株改良至关重要。在本综述中，总结了一种利用射频大气压辉光放电（RF APGD）等离子体射流进行微生物诱变育种的新诱变方法。基于实验研究，已发现氦气RF APGD等离子体射流能够显著改变DNA序列，这表明RF APGD等离子体射流凭借其诸如低且可控的气体温度、丰富的化学反应性物种、快速突变、高操作灵活性等突出特性，将成为微生物诱变的有力工具。随后，以RF APGD等离子体发生器为核心组件，开发了一种名为常压室温等离子体（ARTP）诱变系统的诱变机器，并成功应用于包括细菌、真菌和微藻在内的40多种微生物的诱变育种。最后，讨论了ARTP诱变的前景。
