[牙髓卟啉单胞菌在原发性和继发性牙髓感染中的定植]
[Colonization of Porphyromonas endodontalis in primary and secondary endodontic infections].
作者信息
Hong Li, Hai Ji, Yan-Yan He, Shenghui Yang, Benxiang Hou
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2015 Feb;33(1):88-92. doi: 10.7518/hxkq.2015.01.020.
OBJECTIVE
This study aims to assess and compare the prevalence of Porphyromonas endodontalis (P. endodontalis) in root canals associated with primary and secondary endodontic infections by using 16s rDNA PCR and real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR).
METHODS
A total of 120 adult patients with one radiographically documented periapical lesion were included. Sixty teeth presented with primary endodontic infections and 60 with secondary endodontic infections requiring retreatment. P. endodontalis was identified by using 16s rDNA PCR techniques. The positive DNA expression of P. endodontalis in two types of infected root canals were quantitatively compared by using SYBR GREEN I RTFQ-PCR.
RESULTS
The prevalence of P. endodontalis in the root canals with primary endodontic infections was significantly higher than that in root canals with secondary endodontic infections (P = 0.001). However, RTFQ-PCR results showed no significant difference in DNA expression quantities between the primary and secondary endodontic infections root canals (P = 0.303).
CONCLUSION
P. endodontalis is more highly associated with root canals having primary endodontic infections, although P. endodontalis colonize in both root canals with primary and secondary chronic apical periodontitis.
目的
本研究旨在通过16s rDNA聚合酶链反应（PCR）和实时荧光定量聚合酶链反应（RTFQ-PCR）评估和比较牙髓卟啉单胞菌（牙髓卟啉菌）在与原发性和继发性牙髓感染相关的根管中的患病率。
方法
共纳入120例有根尖周病变影像学记录的成年患者。60颗牙齿患有原发性牙髓感染，60颗牙齿患有需要再治疗的继发性牙髓感染。采用16s rDNA PCR技术鉴定牙髓卟啉菌。利用SYBR GREEN I RTFQ-PCR定量比较两种感染根管中牙髓卟啉菌的阳性DNA表达。
结果
原发性牙髓感染根管中牙髓卟啉菌的患病率显著高于继发性牙髓感染根管（P = 0.001）。然而，RTFQ-PCR结果显示原发性和继发性牙髓感染根管之间的DNA表达量无显著差异（P = 0.303）。
结论
牙髓卟啉菌与原发性牙髓感染的根管相关性更高，尽管牙髓卟啉菌在原发性和继发性慢性根尖周炎的根管中均有定植。
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