Alarfaj Nawal A, El-Tohamy Maha F
Department of Chemistry, College of Science, King Saud University, Riyadh, 11495, Saudi Arabia.
General Administrative of Medical Affairs, Zagazig University, Egypt.
Luminescence. 2015 Dec;30(8):1403-8. doi: 10.1002/bio.2914. Epub 2015 Apr 14.
Two novel sensitive sequential injection chemiluminescence analysis and fluorescence methods for trovafloxacin mesylate detection have been developed. The methods were based on the enhancement effect of gold nanoparticles on luminol-ferricyanide-trovafloxacin and europium(III)-trovafloxacin complex systems. The optimum conditions for both detection methods were investigated. The chemiluminescence signal was emitted due to the enhanced effect of gold nanoparticles on the reaction of luminol-ferricyanide-trovafloxacin in an alkaline medium. The response was linear over a concentration range of 1.0 × 10(-9) to 1.0 × 10(-2) mol/L (%RSD = 1.3), (n = 9, r = 0.9991) with a detection limit of 1.7 × 10(-10) mol/L (S/N = 3). The weak fluorescence intensity signal of the oxidation complex of europium(III)-trovafloxacin was strongly enhanced by gold nanoparticles and detected at λex = 330 and λem = 540 nm. Fluorescence detection enabled the determination of trovafloxacin mesylate over a linear range of 1.0 × 10(-8) to 1.0 × 10(-3) mol/L (%RSD = 1.2), (n = 6, r = 0.9993) with a detection limit of 3.3 × 10(-9) mol/L. The proposed methods were successfully applied to the determination of the studied drug in its bulk form and in pharmaceutical preparations. The results were treated statistically and compared with those obtained from other reported methods.
已开发出两种用于检测甲磺酸妥氟沙星的新型灵敏的顺序注射化学发光分析和荧光方法。这些方法基于金纳米颗粒对鲁米诺-铁氰化物-妥氟沙星和铕（III）-妥氟沙星络合物体系的增强作用。研究了两种检测方法的最佳条件。在碱性介质中，由于金纳米颗粒对鲁米诺-铁氰化物-妥氟沙星反应的增强作用，产生了化学发光信号。在1.0×10（-9）至1.0×10（-2）mol/L的浓度范围内响应呈线性（%RSD = 1.3），（n = 9，r = 0.9991），检测限为1.7×10（-10）mol/L（S/N = 3）。铕（III）-妥氟沙星氧化络合物的弱荧光强度信号被金纳米颗粒强烈增强，并在λex = 330和λem = 540 nm处检测到。荧光检测能够在1.0×10（-8）至1.0×10（-3）mol/L的线性范围内测定甲磺酸妥氟沙星（%RSD = 1.2），（n = 6，r = 0.9993），检测限为3.3×10（-9）mol/L。所提出的方法已成功应用于测定原料药和药物制剂中所研究的药物。对结果进行了统计学处理，并与其他报道方法获得的结果进行了比较。
