Larsen P J, O'Hare M M, Vangsted A, Mikkelsen J D
Department B, University of Copenhagen, Denmark.
Peptides. 1989 Jul-Aug;10(4):815-8. doi: 10.1016/0196-9781(89)90119-8.
Immunohistochemical and chromatographic studies were performed on the guinea pig anterior pituitary gland with an antiserum recognizing an epitope within the gastrin releasing peptide (GRP) carboxyterminal amino acid sequence Val-Gly-His-Leu-Met-NH2. Within the anterior pituitary gland GRP-like immunoreactive cells were identified. The GRP-like immunoreactive cells were distributed heterogenously in the gland, predominantly located in ventral aspects of the anterior pituitary. Intracellularly, the immunoreactivity elements were identified as granula-like structures in the cytoplasma. To further characterize the peptide displaying GRP-like immunoreactivity within the pituitary cells, the GRP-like substances were analyzed by radioimmunoassay and gel filtration chromatography. Using this analytical approach it was determined that the guinea pig pituitary extract contained a peptide with characteristics similar to that of authentic porcine GRP(1-27). Only trace amounts of smaller C-terminal fragments were identified. These results indicate, in contrast to findings in other tissues, the GRP(1-27) is not further degraded into smaller peptide fragments.
用一种识别胃泌素释放肽（GRP）羧基末端氨基酸序列Val-Gly-His-Leu-Met-NH2内表位的抗血清，对豚鼠垂体前叶进行了免疫组织化学和色谱研究。在垂体前叶内鉴定出了GRP样免疫反应性细胞。GRP样免疫反应性细胞在腺体内分布不均，主要位于垂体前叶的腹侧。在细胞内，免疫反应性成分被鉴定为细胞质中的颗粒样结构。为了进一步表征垂体细胞内显示GRP样免疫反应性的肽，通过放射免疫分析和凝胶过滤色谱法对GRP样物质进行了分析。使用这种分析方法确定，豚鼠垂体提取物含有一种具有与正宗猪GRP(1-27)相似特征的肽。仅鉴定出痕量的较小C末端片段。这些结果表明，与其他组织中的发现相反，GRP(1-27)不会进一步降解为更小的肽片段。
