Matsubayashi S, Yanaihara C, Ohkubo M, Fukata S, Hayashi Y, Tamai H, Nakagawa T, Miyauchi A, Kuma K, Abe K
Cancer. 1984 Jun 1;53(11):2472-7. doi: 10.1002/1097-0142(19840601)53:11<2472::aid-cncr2820531118>3.0.co;2-a.
Two cases of gastrin releasing peptide (GRP)-producing medullary thyroid carcinoma are presented. Immunohistochemical examination revealed the presence of GRP-like immunoreactivity (IR-GRP) in the primary tumor tissues. High concentration of IR-GRP was also demonstrated in extracts of the primary tumors by radioimmunologic means with use of a GRP-specific antiserum. Chromatographic analysis showed that the immunoreactivity was composed of at least two molecular forms: one behaved as synthetic porcine GRP on Sephadex G-50 gel filtration and the other as porcine GRP (14-27), a C-terminal active fragment of GRP. The IR-GRP was shown not to be attributed to bombesin-like immunoreactivity. Substance P-like immunoreactivity was not detected in the tumor tissues by either immunohistochemical or radioimmunologic means. This is, as far as the authors are aware, the first finding of IR-GRP as an ectopic product in medullary carcinoma.
本文报告了2例产生胃泌素释放肽（GRP）的甲状腺髓样癌。免疫组织化学检查显示，原发性肿瘤组织中存在GRP样免疫反应性（IR-GRP）。通过使用GRP特异性抗血清的放射免疫方法，在原发性肿瘤提取物中也证实了高浓度的IR-GRP。色谱分析表明，免疫反应性至少由两种分子形式组成：一种在Sephadex G-50凝胶过滤中表现为合成猪GRP，另一种表现为猪GRP（14-27），即GRP的C末端活性片段。IR-GRP被证明不归因于蛙皮素样免疫反应性。通过免疫组织化学或放射免疫方法在肿瘤组织中均未检测到P物质样免疫反应性。据作者所知，这是首次发现IR-GRP作为甲状腺髓样癌中的异位产物。
