猪血管活性肠肽在舒张豚鼠子宫动脉方面比豚鼠自身的血管活性肠肽更有效。

Porcine VIP is more potent than guinea pig VIP in relaxing the guinea pig uterine artery.

作者信息

Morris J L, Murphy R

机构信息

Department of Anatomy and Histology, School of Medicine, Flinders University of South Australia, Bedford Park.

出版信息

Peptides. 1989 Jul-Aug;10(4):887-9. doi: 10.1016/0196-9781(89)90130-7.

DOI:10.1016/0196-9781(89)90130-7

PMID:2587428

Abstract

The vasodilator potency of guinea pig VIP (gp VIP) on the guinea pig uterine artery was compared with the potency of porcine VIP (p VIP), which differs in amino acid sequence at four locations. When antioxidants were not used, the two peptides were approximately equipotent in causing relaxation of precontracted vessel segments. Use of the antioxidants ascorbic acid and dithiothreitol resulted in significantly increased potency of both peptides. Porcine VIP was 15 times more potent than gp VIP synthesized by the same method (tBoc), and gp VIP synthesized by tBoc methodology was 2 times more potent than gp VIP synthesized by Fmoc methodology. Therefore, care should be taken in the choice and handling of synthetic peptides when aiming to mimic actions of endogenous peptides.

摘要

将豚鼠血管活性肠肽（gp VIP）对豚鼠子宫动脉的血管舒张效力与猪血管活性肠肽（p VIP）的效力进行了比较，这两种肽在四个位置的氨基酸序列不同。当不使用抗氧化剂时，这两种肽在使预收缩血管段舒张方面效力大致相当。使用抗氧化剂抗坏血酸和二硫苏糖醇导致两种肽的效力显著增加。通过相同方法（叔丁氧羰基）合成的猪血管活性肠肽的效力比豚鼠血管活性肠肽强15倍，通过叔丁氧羰基方法合成的豚鼠血管活性肠肽的效力比通过芴甲氧羰基方法合成的豚鼠血管活性肠肽强2倍。因此，在旨在模拟内源性肽的作用时，选择和处理合成肽时应谨慎。