Glinka Elena M
, Voljskii bulvar 95, 3/244, Moscow, 109125, Russia,
Tumour Biol. 2015 May;36(5):3147-57. doi: 10.1007/s13277-015-3360-z. Epub 2015 Apr 1.
Cancer gene therapy vectors are promising tools for killing cancer cells with the purpose of eradicating malignant tumours entirely. Different delivery methods of vectors into the cancer cells, including both non-viral and viral, as well as promoters for the targeted expression of genes encoding anticancer proteins were developed for effective and selective killing of cancer cells without harming healthy cells. Many vectors have been created to kill cancer cells, and some vectors suppress malignant tumours with high efficiency. This review is focused on vectors bearing genes for nucleases such as deoxyribonucleases (caspase-activated DNase, deoxyribonuclease I-like 3, endonuclease G) and ribonucleases (human polynucleotide phosphorylase, ribonuclease L, α-sarcin, barnase), as well as vectors harbouring gene encoding ribonuclease inhibitor. The data concerning the functionality and the efficacy of such vectors are presented.
癌症基因治疗载体是有望彻底根除恶性肿瘤的杀死癌细胞的工具。为了在不损害健康细胞的情况下有效且选择性地杀死癌细胞，人们开发了多种将载体导入癌细胞的方法，包括非病毒和病毒方法，以及用于靶向表达编码抗癌蛋白的基因的启动子。已经创建了许多用于杀死癌细胞的载体，并且一些载体能够高效抑制恶性肿瘤。本综述聚焦于携带核酸酶基因的载体，如脱氧核糖核酸酶（半胱天冬酶激活的脱氧核糖核酸酶、脱氧核糖核酸酶I样3、核酸内切酶G）和核糖核酸酶（人多聚核苷酸磷酸化酶、核糖核酸酶L、α-肌动蛋白、芽孢杆菌RNA酶），以及携带编码核糖核酸酶抑制剂基因的载体。文中呈现了关于此类载体的功能和功效的数据。
