厌氧一氧化碳氧化菌微生物群落的宏基因组分析。
Metagenomic analysis for the microbial consortium of anaerobic CO oxidizers.
作者信息
Guo Ying, Xu Jingliang, Yuan Zhenhong, Li Xiekun, Zhou Weizheng, Xu Huijuan, Liang Cuiyi, Zhang Yu, Zhuang Xinshu
机构信息
Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, China.
Graduate University of Chinese Academy of Sciences, Beijing, 100049, China.
出版信息
Microb Biotechnol. 2015 Sep;8(5):846-52. doi: 10.1111/1751-7915.12283. Epub 2015 Apr 15.
Metagenomics analysis has been applied to identify the dominant anaerobic microbial consortium of the carbon monoxide (CO) oxidizers in anaerobic sludge. Reads from the hypervariable V6 region in the bacterial 16s rDNA were aligned and finally clustered into operational taxonomic units (OTUs). The OTUs from different stages in anaerobic CO condition were classified. Alphaproteobacteria, clostridia, betaproteobacteria and actinobacteria were the most abundant groups, while alphaproteobacteria, betaproteobacteria and actinobacteria were variable groups. CO consumption and production efficiency of the microbial consortium were studied. Semi-continuous trials showed that these anaerobic CO oxidizers formed a stable microbial community, and the CO conversion rate was at over 84%, with the highest CO consumption activity of 28.9 mmol CO/g VSS●day and methane production activity at 7.6 mmol CH₄ /g VSS●day during six cycles.
宏基因组学分析已被用于鉴定厌氧污泥中一氧化碳（CO）氧化菌的主要厌氧微生物群落。对细菌16s rDNA高变V6区域的读数进行比对，最终聚类为操作分类单元（OTU）。对厌氧CO条件下不同阶段的OTU进行了分类。α-变形菌纲、梭菌纲、β-变形菌纲和放线菌是最丰富的类群，而α-变形菌纲、β-变形菌纲和放线菌是可变类群。研究了微生物群落的CO消耗和生产效率。半连续试验表明，这些厌氧CO氧化菌形成了一个稳定的微生物群落，CO转化率超过84%，在六个周期中，最高CO消耗活性为28.9 mmol CO/g VSS●天，甲烷产生活性为7.6 mmol CH₄ /g VSS●天。
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